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首页> 外文期刊>The European Journal of Neuroscience >Neural cell adhesion molecule is endocytosed via a clathrin-dependent pathway.
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Neural cell adhesion molecule is endocytosed via a clathrin-dependent pathway.

机译：神经细胞粘附分子通过网格蛋白依赖性途径被内吞。

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摘要

Neural cell adhesion molecule (NCAM) constitutes a group of cell surface glycoproteins that regulate cell-cell interactions in the developing and adult brain. Endocytosis is a mechanism which dynamically controls the amount of cell surface NCAM expression and may involve the rapid changes occurring in NCAM expression under certain physiological or pathological conditions. However, the endocytic pathway of NCAM is presently unknown. Using astrocytes in culture and immunofluorescence we show that NCAM is internalized and that the immunolabelling presents a high degree of colocalization with clathrin, alpha-adaptin and transferrin, suggesting that NCAM is endocytosed by a clathrin-dependent pathway. Potassium depletion which disrupts clathrin-mediated endocytosis, inhibited internalization of NCAM. Electron microscopy and immunogold studies also demonstrate that the surface of clathrin-coated vesicles are also immunolabelled for both alpha-adaptin and PSA-NCAM, the highly sialylated isoform of NCAM. Furthermore, immunoprecipation studies demonstrate that NCAM is associated with both clathrin and alpha-adaptin, a component of adaptor complex AP-2, in brain, neurons and astrocytes. These findings indicate that NCAM is mainly endocytosed via clathrin-coated vesicles, suggesting a possible mechanism that may contribute to the rapid changes in NCAM expression at the cell surface.
机译：神经细胞粘附分子（NCAM）构成了一组调节发育中和成年大脑中细胞与细胞相互作用的细胞表面糖蛋白。内吞作用是一种动态控制细胞表面NCAM表达量的机制，可能涉及在某些生理或病理条件下NCAM表达发生的快速变化。但是，目前尚不清楚NCAM的内吞途径。在培养和免疫荧光实验中使用星形胶质细胞，我们显示NCAM被内在化，并且免疫标记与网格蛋白，α-adaptin和转铁蛋白呈现高度共定位，表明NCAM被网格蛋白依赖性途径内吞。破坏网格蛋白介导的内吞作用的钾耗竭抑制了NCAM的内在化。电子显微镜和免疫金研究还表明，网格蛋白包被的囊泡表面也被免疫标记了α-适配蛋白和PSA-NCAM（NCAM的高度唾液酸化亚型）。此外，免疫沉淀研究表明，NCAM与脑，神经元和星形胶质细胞中的网格蛋白和α-适体蛋白（适配器复合物AP-2的组成部分）相关。这些发现表明，NCAM主要通过网格蛋白包被的囊泡内吞，提示可能的机制可能有助于细胞表面NCAM表达的快速变化。

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