...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Calcium currents in rat dentate granule cells are altered after adrenalectomy.
【24h】

Calcium currents in rat dentate granule cells are altered after adrenalectomy.

机译：肾上腺切除术后大鼠齿状颗粒细胞中的钙电流发生改变。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Adrenal corticosteroid hormones modulate voltage-gated calcium currents in rat CA1 hippocampal neurons. In the present whole-cell recording study we examined whether calcium currents in dentate granule cells are also under control of corticosteroids. In a first series of experiments, in which the calcium chelator BAPTA was added to the recording solution, the amplitude of calcium currents induced by a voltage step to -10 mV was found to be enhanced shortly (1 or 2 days) after adrenalectomy compared to sham operation. No enhancement was seen when adrenalectomized animals received a low dose of corticosterone in the drinking water. By contrast, 3 or 7 days after adrenalectomy calcium current amplitude was decreased. Starting 3 days after adrenalectomy, some of the granule cells underwent apoptosis. This caused a bias in the recorded cell population towards relatively apoptosis-resistant cells, suggesting that restricted calcium influx may be a key feature of cells withstanding the apoptotic route. In accordance, cells from a small percentage ( approximately 20%) of animals that resisted apoptosis after adrenalectomy also displayed small calcium currents. In a second series without BAPTA, thus focusing on the endogenous calcium-buffering capacity, we found that the time constant for the decay of the calcium current was decreased after adrenalectomy, probably due to enhanced calcium-dependent inactivation of the current. The data indicate that cellular calcium current characteristics of dentate granule cells are altered after adrenalectomy and that the alterations may in part determine the vulnerability to undergo apoptosis.
机译：肾上腺皮质类固醇激素调节大鼠CA1海马神经元的电压门控钙电流。在目前的全细胞记录研究中，我们检查了齿状颗粒细胞中的钙电流是否也处于皮质类固醇的控制之下。在第一个系列实验中，向记录溶液中添加了钙螯合剂BAPTA，与肾上腺切除术相比，在肾上腺切除术后不久（1或2天），电压阶跃感应到-10 mV引起的钙电流幅度被增强。假操作。当肾上腺切除的动物在饮用水中接受低剂量的皮质酮时，未见增强。相比之下，肾上腺切除术后3或7天钙电流幅度降低。肾上腺切除术后3天开始，一些颗粒细胞发生凋亡。这导致所记录的细胞群体偏向于相对抗凋亡的细胞，这表明受限制的钙内流可能是承受凋亡途径的细胞的关键特征。因此，肾上腺切除术后抵抗细胞凋亡的小部分动物（约20％）的细胞也表现出较小的钙流。在没有BAPTA的第二个系列中，因此着眼于内源性钙缓冲能力，我们发现肾上腺切除术后钙电流衰减的时间常数减小了，这可能是由于电流依赖于钙的失活增强所致。数据表明，肾上腺切除术后齿状颗粒细胞的钙离子电流特征发生改变，这种改变可能部分决定了细胞凋亡的易感性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号