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首页> 外文期刊>The European Journal of Neuroscience >Characterization of retinal guanylate cyclase-activating protein 3 (GCAP3) from zebrafish to man.
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Characterization of retinal guanylate cyclase-activating protein 3 (GCAP3) from zebrafish to man.

机译：从斑马鱼到人的视网膜鸟苷酸环化酶激活蛋白3（GCAP3）的表征。

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摘要

Calmodulin-like neuronal Ca2+-binding proteins (NCBPs) are expressed primarily in neurons and contain a combination of four functional and nonfunctional EF-hand Ca2+-binding motifs. The guanylate cyclase-activating proteins 1-3 (GCAP1-3), the best characterized subgroup of NCBPs, function in the regulation of transmembrane guanylate cyclases 1-2 (GC1-2). The pairing of GCAPs and GCs in vivo depends on cell expression. Therefore, we investigated the expression of these genes in retina using in situ hybridization and immunocytochemistry. Our results demonstrate that GCAP1, GCAP2, GC1 and GC2 are expressed in human rod and cone photoreceptors, while GCAP3 is expressed exclusively in cones. As a consequence of extensive modification, the GCAP3 gene is not expressed in mouse retina. However, this lack of evolutionary conservation appears to be restricted to only some species as we cloned all three GCAPs from teleost (zebrafish) retina and localized them to rod cells, short single cones (GCAP1-2), and all subtypes of cones (GCAP3). Furthermore, sequence comparisons and evolutionary trace analysis coupled with functional testing of the different GCAPs allowed us to identify the key conserved residues that are critical for GCAP structure and function, and to define class-specific residues for the NCBP subfamilies.
机译：钙调蛋白样神经元Ca2 +结合蛋白（NCBPs）主要在神经元中表达，并包含四个功能性和非功能性EF手Ca2 +结合基序的组合。鸟苷酸环化酶激活蛋白1-3（GCAP1-3）是NCBPs中最具特征的亚组，在调节跨膜鸟苷酸环化酶1-2（GC1-2）中起作用。体内GCAP和GC的配对取决于细胞表达。因此，我们使用原位杂交和免疫细胞化学研究了这些基因在视网膜中的表达。我们的结果表明，GCAP1，GCAP2，GC1和GC2在人的视锥细胞和视锥细胞中表达，而GCAP3仅在视锥细胞中表达。作为广泛修饰的结果，GCAP3基因不在小鼠视网膜中表达。但是，这种缺乏进化保守性的现象似乎仅限于某些物种，因为我们从硬骨鱼（斑马鱼）视网膜中克隆了所有三个GCAP，并将它们定位于杆状细胞，短单锥（GCAP1-2）和所有锥的亚型（GCAP3）。 ）。此外，序列比较和进化痕量分析与不同GCAP的功能测试相结合，使我们能够确定对GCAP结构和功能至关重要的关键保守残基，并为NCBP亚家族定义特定于类的残基。

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