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首页> 外文期刊>The European Journal of Neuroscience >Ionic currents determining the membrane characteristics of type I spiral ganglion neurons of the guinea pig.
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Ionic currents determining the membrane characteristics of type I spiral ganglion neurons of the guinea pig.

机译：离子流决定了豚鼠I型螺旋神经节神经元的膜特征。

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Enzymatically isolated type I spiral ganglion neurons of the guinea pig have been investigated in the present study. The identity of the cells was confirmed by using anti-neuron-specific enolase immunostaining. The presence and shredding of the myelin sheath was also documented by employing anti-S100 immunoreaction. The membrane characteristics of the cells were studied by using the whole-cell patch-clamp technique. The whole-cell capacitance of the cells was 9 +/- 2 pF (n = 51), while the resting membrane potential of the cells was -62 +/- 9 mV (n = 19). When suprathreshold depolarizing stimuli were applied, the neurons fired a single action potential at the beginning of the stimulation. It was confirmed in this study that type I spiral ganglion cells possess a hyperpolarization-activated nonspecific cationic current (Ih). The major characteristics of this current component were unaffected by the enzyme treatment. Type I spiral ganglion cells also expressed various depolarization-activated K+ current components. A high-threshold outward current was sensitive to 1-10 mm TEA+ application. The ganglion cells also expressed a relatively small, but nevertheless present, transient outward current component which was less sensitive to TEA+ but could be inhibited by 100 micro m 4-aminopyridine. A DTX-I-sensitive current was responsible for some 30% of the total outward current (at 0 mV), showed rapid activation at membrane potentials positive to -50 mV and demonstrated very little inactivation. However, inhibition of the highly 4-AP- or DTX-I-sensitive component did not alter the rapidly inactivating nature of the firing pattern of the cells.
机译：在本研究中已经研究了豚鼠的酶促分离的I型螺旋神经节神经元。通过使用抗神经元特异性烯醇酶免疫染色确认了细胞的身份。还通过采用抗S100免疫反应记录了髓鞘的存在和切碎。通过使用全细胞膜片钳技术研究细胞的膜特性。细胞的全细胞电容为9 +/- 2 pF（n = 51），而细胞的静息膜电位为-62 +/- 9 mV（n = 19）。当施加阈上去极化刺激时，神经元在刺激开始时会激发单个动作电位。在这项研究中证实，I型螺旋神经节细胞具有超极化激活的非特异性阳离子电流（Ih）。该当前组分的主要特征不受酶处理的影响。 I型螺旋神经节细胞还表达了各种去极化激活的K +电流成分。高阈值向外电流对1-10 mm TEA +应用敏感。神经节细胞还表达相对较小但仍然存在的瞬时外向电流成分，该成分对TEA +较不敏感，但可以被100微米的4-氨基吡啶抑制。 DTX-I敏感电流约占总向外电流的30％（在0 mV时），在正电位至-50 mV的膜电位下显示出快速活化，并且几乎没有失活。但是，抑制高度4-AP-或DTX-1敏感的成分不会改变细胞放电模式的快速失活性质。

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