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首页> 外文期刊>The European Journal of Neuroscience >Melanopsin retinal ganglion cells and the maintenance of circadian and pupillary responses to light in aged rodless/coneless (rd/rd cl) mice.
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Melanopsin retinal ganglion cells and the maintenance of circadian and pupillary responses to light in aged rodless/coneless (rd/rd cl) mice.

机译：在衰老的无杆/无锥（rd / rd cl）小鼠中，黑素蛋白视网膜神经节细胞和对光的昼夜节律和瞳孔反应的维持。

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Melanopsin-expressing ganglion cells have been proposed as the photoreceptors mediating non-rod, non-cone ocular responses to light. Here we use the aged (approximately 2 years) rodless and coneless (rd/rd cl) mouse to assess the impact of progressive inner retinal cell loss on melanopsin expression, circadian entrainment and pupillary constriction. Aged rd/rd cl mice show substantial transneuronal retinal degeneration leaving only the ganglion cell layer and little of the inner nuclear layer. Despite this loss, quantitative reverse transcriptase-polymerase chain reaction showed normal levels of melanopsin expression, and immunocytochemistry demonstrated both the presence and normal cellular appearance of these cells. Furthermore, the optic nerves of the two genotypes (rd/rd cl and +/+) were not obviously different in animals older than 2 years. However, this massive level of retinal degeneration left both pupillary and circadian responses to light intact, even in rd/rd cl mice older than 2 years. Our data provide the first positive correlation between the persistence of melanopsin-expressing cells and the maintenance of both circadian and pupillary responses to light in the absence of rods and cones. These findings, together with recent studies on melanopsin knockout mice, are consistent with the hypothesis that melanopsin-expressing ganglion cells are photosensitive and mediate a range of irradiance-detection tasks.
机译：已经提出了表达黑素蛋白的神经节细胞作为介导对光的无杆，无锥眼反应的感光体。在这里，我们使用老年（约2年）无杆和无锥（rd / rd cl）小鼠评估渐进性视网膜内部细胞丢失对黑素表达，昼夜节律夹带和瞳孔收缩的影响。老年rd / rd cl小鼠表现出明显的跨神经元视网膜变性，仅留下神经节细胞层，几乎没有内核层。尽管有这种损失，定量逆转录酶-聚合酶链反应仍显示正常水平的黑素表达，而免疫细胞化学表明这些细胞的存在和正常细胞外观。此外，在2岁以上的动物中，两种基因型（rd / rd cl和+ / +）的视神经没有明显差异。然而，即使在年龄大于2岁的rd / rd cl小鼠中，这种大量的视网膜变性也使瞳孔和昼夜节律对光保持完整。我们的数据提供了黑色素表达细胞的持久性与在没有杆和锥的情况下维持昼夜节律和瞳孔对光的反应之间的第一个正相关性。这些发现以及最近对黑视蛋白敲除小鼠的研究，与表达黑视蛋白的神经节细胞具有光敏性并介导一系列辐照检测任务的假设相一致。

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