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首页> 外文期刊>The European Journal of Neuroscience >Reduced food anticipatory activity in genetically orexin (hypocretin) neuron-ablated mice.
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Reduced food anticipatory activity in genetically orexin (hypocretin) neuron-ablated mice.

机译：遗传性食欲素（hypocretin）神经元切除小鼠的食物预期活性降低。

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摘要

Daily restricted feeding (RF) produces an anticipatory locomotor activity rhythm and entrains the peripheral molecular oscillator independently of the central pacemaker located in the suprachiasmatic nucleus (SCN). As orexins (hypocretins) are neuropeptides that coordinate sleep/wake patterns and motivated behaviours, such as food seeking, we studied the involvement of orexin in the food anticipatory activity (FAA) induced by RF. Daily RF shifted the mRNA rhythm of a clock-controlled gene mDbp in the cerebral cortex and caudate putamen but not in the SCN. Under these experimental conditions, prepro-orexin mRNA and orexin A immunoreactivity in the lateral hypothalamic area (LHA) did not show daily variation. Fasting increased the number of orexin A-ir cells, while RF did not. However, RF shifted the peak of Fos expression of the orexin neurons from night to day. Genetic ablation of orexin neurons in orexin/ataxin-3 transgenic mice severely reduced the formation of FAA under RF conditions. The expressionof mNpas2 mRNA, a transcription factor thought to be involved in regulation of the food entrainable oscillator as well as mPer1 and mBmal1 mRNA, was reduced in the forebrain of orexin/ataxin-3 mice. Based on these results, we suggest that activity of the orexin neuron in the LHA contributes to the promotion and maintenance of FAA.
机译：每日限制进食（RF）产生预期的运动活动节律，并独立于位于视交叉上核（SCN）的中央起搏器带动周围的分子振荡器。由于食欲肽（hypocretins）是协调睡眠/唤醒方式和动机行为（例如寻找食物）的神经肽，因此我们研究了食欲肽在RF诱导的食物预期活动（FAA）中的参与。每日RF改变了大脑皮层和尾状壳中的时钟控制基因mDbp的mRNA节律，但SCN中却没有。在这些实验条件下，下丘脑外侧区（LHA）的前原毒素mRNA和食欲素A免疫反应性没有显示出每日变化。空腹增加了食欲素A-ir细胞的数量，而RF没有。但是，RF将食欲素神经元的Fos表达高峰从夜晚转移到了白天。 Orexin / ataxin-3转基因小鼠中orexin神经元的遗传消融严重降低了RF条件下FAA的形成。在orexin / ataxin-3小鼠的前脑中，mNpas2 mRNA的表达降低了，mNpas2 mRNA是一种被认为与食物可携带振荡器以及mPer1和mBmal1 mRNA调控有关的转录因子。根据这些结果，我们建议LHA中的orexin神经元的活性有助于促进和维持FAA。

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