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首页> 外文期刊>The European Journal of Neuroscience >GABA and CB cannabinoid receptor expression identifies two types of septal cholinergic neurons.
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GABA and CB cannabinoid receptor expression identifies two types of septal cholinergic neurons.

机译：GABA和CB大麻素受体表达鉴定出两种类型的间隔胆碱能神经元。

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Abstract Septohippocampal cholinergic neurons play key roles in learning and memory processes, and in the generation of hippocampal theta rhythm. The range of receptors for endogenous modulators expressed on these neurons is unclear. Here we describe GABA(B) 1a/b receptor (GABA(B)R) and type 1 cannabinoid receptor (CB(1)R) expression in rat septal cholinergic [i.e. choline acetyltransferase (ChAT)-positive] cells. Using double immunofluorescent staining, we found that almost two-thirds of the cholinergic cells in the rat medial septum were GABA(B)R positive, and that these cells had significantly larger somata than did GABA(B)R-negative cholinergic neurons. We detected CB(1)R labelling in somata after axonal protein transport was blocked by colchicine. In these animals about one-third of the cholinergic cells were CB(1)R positive. These cells again had larger somata than CB(1)R-negative cholinergic neurons. The analyses confirmed that the size of GABA(B)R-positive and CB(1)R-positive cholinergic cells were alike, and all CB(1)R-positive cholinergic cells were GABA(B)R positive as well. CB(1)R-positive cells were invariably ChAT positive. All retrogradely labelled septohippocampal cholinergic cells were positive for GABA(B)R and at least half of them also for CB(1)R. These data shed light on the existence of at least two cholinergic cell types in the medial septum: one expresses GABA(B)R and CB(1)R, has large somata and projects to the hippocampus, whereas the other is negative for GABA(B)R and CB(1)R and has smaller somata. The results also suggest that cholinergic transmission in the hippocampus is fine-tuned by endocannabinoid signalling.
机译：摘要海马区胆碱能神经元在学习和记忆过程以及海马θ节律的产生中起关键作用。这些神经元上表达的内源性调节剂的受体范围尚不清楚。在这里，我们描述了大鼠中隔胆碱能的GABA（B）1a / b受体（GABA（B）R）和1型大麻素受体（CB（1）R）表达。胆碱乙酰转移酶（ChAT）阳性]细胞。使用双重免疫荧光染色，我们发现大鼠中隔中的胆碱能细胞中几乎有三分之二是GABA（B）R阳性，并且这些细胞的体细胞比GABA（B）R阴性的胆碱能神经元大得多。秋水仙碱阻止轴突蛋白运输后，我们在躯体中检测到CB（1）R标记。在这些动物中，大约三分之一的胆碱能细胞是CB（1）R阳性的。这些细胞再次比CB（1）R阴性胆碱能神经元具有更大的躯体。分析证实，GABA（B）R阳性胆碱能细胞和CB（1）R阳性胆碱能细胞的大小相同，并且所有CB（1）R阳性胆碱能细胞也均为GABA（B）R阳性。 CB（1）R阳性细胞始终为ChAT阳性。所有逆行标记的海马胆碱能细胞均对GABA（B）R呈阳性，并且至少一半对CB（1）R也呈阳性。这些数据揭示了内侧隔膜中至少存在两种​​胆碱能细胞类型：一种表达GABA（B）R和CB（1）R，具有较大的躯体细胞并向海马突出，而另一种则对GABA呈阴性。 B）R和CB（1）R，且躯体较小。该结果还表明，通过内源性大麻素信号微调海马中的胆碱能传递。

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