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首页> 外文期刊>The European Journal of Neuroscience >Differential effects of trans-crotononitrile and 3-acetylpyridine on inferior olive integrity and behavioural performance in the rat.
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Differential effects of trans-crotononitrile and 3-acetylpyridine on inferior olive integrity and behavioural performance in the rat.

机译：反式巴豆腈和3-乙酰基吡啶对大鼠下橄榄完整性和行为表现的差异作用。

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摘要

The inferior olive climbing fibre projection is key to cerebellar contributions to motor control. Here we present evidence for a novel tool, trans-crotononitrile (TCN), to selectively inactivate the olive to study its functions. Anatomical, electrophysiological and behavioural techniques have been used in rats to assess the CNS effects of TCN, with a focus on the olivocerebellar projection. These findings were compared with those obtained with 3-acetylpyridine (plus nicotinamide administered 3.5 h later, 3AP + 3.5 h). Fluoro-Jade B cell labelling showed that TCN and 3AP + 3.5 h induce neurodegeneration primarily within the inferior olive, with no other targets in common. Recordings of evoked field potentials on the cerebellar cortical surface showed that both neurotoxins can reduce transmission in climbing fibre but not mossy fibre pathways. Both histological and electrophysiological differences suggest that TCN and 3AP have distinct mechanisms of action. Estimates of the numbers of surviving cells within individual subdivisions of the olive indicate that TCN and 3AP + 3.5 h cause different patterns of subtotal olivary lesion: most surviving neurons are present in the rostral (TCN) or caudal (3AP + 3.5 h) parts of the medial accessory olive, which are associated with two different cerebellar modules: the C2 and A modules, respectively. In behavioural studies, TCN and 3AP + 3.5 h produced differences in motor deficits consistent with the notion that these cerebellar modules have distinct functional responsibilities. Thus, studies using TCN as compared with 3AP + 3.5 h have the potential to shed light on the contributions of different cerebellar modules in motor control.
机译：橄榄色攀爬纤维下方投射是小脑对运动控制的贡献的关键。在这里，我们为新的工具反式巴豆腈（TCN）提供证据，以选择性地灭活橄榄以研究其功能。解剖，电生理和行为技术已在大鼠中用于评估TCN的中枢神经系统作用，重点是小脑小脑投射。将这些发现与使用3-乙酰基吡啶（加上3.5小时后的3AP + 3.5小时施用烟酰胺）获得的结果进行比较。 Fluoro-Jade B细胞标记显示，TCN和3AP + 3.5 h主要在下橄榄中诱导神经变性，没有其他共同目标。小脑皮层表面诱发的电场电位的记录表明，两种神经毒素都可以减少在攀爬纤维中的传输，但不能减少生苔纤维的传输。组织学和电生理学差异均表明TCN和3AP具有不同的作用机制。对橄榄各个子区域中存活细胞的数量的估计表明，TCN和3AP + 3.5 h引起了小卵次病变的不同模式：大多数存活的神经元存在于橄榄的喙部（TCN）或尾部（3AP + 3.5 h）部分内侧副橄榄，分别与两个不同的小脑模块：C2和A模块相关。在行为研究中，TCN和3AP + 3.5 h产生了运动缺陷的差异，这与这些小脑模块具有不同的功能职责的观念一致。因此，与3AP + 3.5 h相比，使用TCN进行的研究有可能阐明不同小脑模块在运动控制中的作用。

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