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首页> 外文期刊>The European Journal of Neuroscience >Inositol 1,4,5-trisphosphate and adenophostin analogues induce responses in turtle olfactory sensory neurons.
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Inositol 1,4,5-trisphosphate and adenophostin analogues induce responses in turtle olfactory sensory neurons.

机译：肌醇1,4,5-三磷酸和腺苷类似物诱导龟嗅感觉神经元的反应。

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摘要

Using the whole-cell mode of the patch-clamp technique, we recorded inward currents in response to inositol-1,4,5-trisphosphate (IP3) and adenophostin analogues in turtle olfactory sensory neurons. Dialysis of IP3 into the neurons induced inward currents with an increase in membrane conductance in a dose-dependent manner under the voltage-clamp conditions (holding potential -70 mV). The application of Ca2+-free Ringer solution to neurons previously dialysed with IP3 induced inward currents that were reversibly inhibited by application of Na+, Ca2+-free Ringer solution, normal Ringer solution or 10 microM ruthenium red. Dialysis of the adenophostin analogues, novel IP3 receptor ligands, also induced inward currents with an increase in membrane conductance. The magnitude of the responses to the adenophostin analogues varied among these derivatives. The application of Ca2+-free Ringer solution to neurons previously dialysed with the adenophostin analogues induced inward currents that were inhibited by the application of normal Ringer solution. The reversal potential of inward currents induced by an adenophostin analogue was similar to that induced by IP3, suggesting that inward currents induced by the adenophostin analogue were generated by a similar ionic mechanism to that induced by IP3. The present study demonstrated that IP3-mediated transduction pathways exist in turtle olfactory receptor neurons and that adenophostin analogues act as agonists of IP3.
机译：使用膜片钳技术的全细胞模式，我们记录了海龟嗅觉感觉神经元中响应肌醇-1,4,5-三磷酸（IP3）和腺苷类似物的内向电流。在电压钳制条件下（保持电位为-70 mV），将IP3渗入神经元，诱导内向电流，并以剂量​​依赖性方式增加膜电导。将不含Ca2 +的林格溶液应用于先前用IP3诱导的内向电流透析的神经元，该神经元可通过应用Na +，不含Ca2 +的林格溶液，普通林格溶液或10 microM钌红可逆地抑制。腺磷素类似物（新的IP3受体配体）的透析也会诱导内向电流，并增加膜电导。在这些衍生物之间，对腺磷素类似物的反应程度有所不同。将不含Ca2 +的林格溶液应用到先前用腺磷素类似物透析的神经元上，可以诱导内向电流，而内向电流则受到普通林格溶液的抑制。腺苷类似物诱导的内向电流的逆向电势与IP3诱导的相似，这表明腺苷类似物诱导的内向电流的产生与IP3诱导的离子机制相似。本研究表明，IP3介导的转导通路存在于龟嗅觉受体神经元中，并且腺苷类似物充当IP3的激动剂。

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