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首页> 外文期刊>The European Journal of Neuroscience >Adult neurogenesis in primate and rodent spinal cord: comparing a cervical dorsal rhizotomy with a dorsal column transection.
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Adult neurogenesis in primate and rodent spinal cord: comparing a cervical dorsal rhizotomy with a dorsal column transection.

机译：灵长类动物和啮齿类动物脊髓中的成人神经发生：比较颈背脊柱切开术和背柱横切术。

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Neurogenesis has not been shown in the primate spinal cord and the conditions for its induction following spinal injury are not known. In the first part of this study, we report neurogenesis in the cervical spinal dorsal horn in adult monkeys 6-8 weeks after receiving a well-defined cervical dorsal rhizotomy (DRL). 5-bromo-2-deoxyuridine (BrdU) was administered 2-4 weeks following the lesion. Cells colabeled with BrdU and five different neuronal markers were observed in the peri-lesion dorsal horn 4-5 weeks after BrdU injection. Those colabeled with BrdU and neuron-specific nuclear protein, and BrdU and glial fibrillary acidic protein were quantified in the dorsal horn peri-lesion region, and the ipsi- and contralateral sides were compared. A significantly greater number of BrdUeuron-specific nuclear protein- and BrdU/glial fibrillary acidic protein-colabeled cells were found on the lesion side (P<0.01). These findings led us to hypothesize that neurogenesis can occur within the spinal cord followinginjury, when the injury does not involve direct trauma to the cord and glial scar formation. This was tested in rats. Neurogenesis and astrocytic proliferation were compared between animals receiving a DRL and those receiving a dorsal column lesion. In DRL rats, neurogenesis was observed in the peri-lesion dorsal horn. In dorsal column lesion rats, no neurogenesis was observed but astrocytic activation was intense. The rat data support our hypothesis and findings in the monkey, and show that the response is not primate specific. The possibility that new neurons contribute to recovery following DRL now needs further investigation.
机译：在灵长类脊髓中尚未显示神经发生，并且在脊髓损伤后诱导其的条件尚不清楚。在这项研究的第一部分中，我们报告了成年猴子在接受明确的颈背背侧切开术（DRL）后的6-8周内颈背背角中的神经发生。病变后2-4周施用5-溴-2-脱氧尿苷（BrdU）。注射BrdU后4-5周，在病变周围的背角中观察到了用BrdU和五种不同的神经元标记物共同标记的细胞。在背角周围病变区域定量标记与BrdU和神经元特异性核蛋白，BrdU和神经胶质原纤维酸性蛋白共标记的那些，并比较同侧和对侧。在病变侧发现明显更多的BrdU /神经元特异性核蛋白和BrdU /神经胶质纤维酸性蛋白共标记细胞（P <0.01）。这些发现使我们假设，损伤不涉及对脊髓的直接损伤和神经胶质瘢痕形成时，神经损伤可能发生在损伤后的脊髓内。在大鼠中对此进行了测试。比较接受DRL的动物和接受背柱病变的动物的神经发生和星形细胞增殖。在DRL大鼠中，在病灶周围背角中观察到神经发生。在背柱病变大鼠中，未观察到神经发生，但星形细胞活化强烈。大鼠的数据支持了我们在猴子中的假设和发现，并表明该反应不是灵长类特异性的。现在，需要进一步研究DRL后新神经元促进恢复的可能性。

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