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首页> 外文期刊>The European Journal of Neuroscience >Temporal profile of circadian clock gene expression in a transplanted suprachiasmatic nucleus and peripheral tissues.
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Temporal profile of circadian clock gene expression in a transplanted suprachiasmatic nucleus and peripheral tissues.

机译：昼夜节律性核和周围组织中昼夜节律基因表达的时间变化。

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The mammalian hypothalamic suprachiasmatic nucleus (SCN) is the master oscillator that regulates the circadian rhythms of the peripheral oscillators. Previous studies have demonstrated that the transplantation of embryonic SCN tissues into SCN-lesioned arrhythmic mice restores the behavioral circadian rhythms of these animals. In our present study, we examined the clock gene expression profiles in a transplanted SCN and peripheral tissues, and also analysed the circadian rhythm of the locomotor activity in SCN-grafted mice. These experiments were undertaken to elucidate whether the transplanted SCN generates a dynamic circadian oscillation and maintains the phase relationships that can be detected in intact mice. The grafted SCN indeed showed dynamic circadian expression rhythms of clock genes such as mPeriod1 (mPer1) and mPeriod2 (mPer2). Furthermore, the phase differences between the expression rhythms of these genes in the grafted SCN and the locomotor activity rhythms of the transplanted animals were found to be very similar to those in intact animals. Moreover, in the liver, kidney and skeletal muscles of the transplanted animals, the phase angles between the circadian rhythm of the grafted SCN and that of the peripheral tissues were maintained as in intact animals. However, in the SCN-grafted animals, the amplitudes of the mPer1 and mPer2 rhythms were attenuated in the peripheral tissues. Our current findings therefore indicate that a transplanted SCN has the capacity to generate a dynamic intrinsic circadian oscillation, and can also lock the normal phase angles among the SCN, locomotor activity and peripheral oscillators in a similar manner as in intact control animals.
机译：哺乳动物的丘脑丘脑上视神经核（SCN）是调节周围振荡器的昼夜节律的主振荡器。先前的研究表明，将胚胎SCN组织移植到SCN病变的心律失常小鼠中可以恢复这些动物的行为昼夜节律。在我们目前的研究中，我们检查了SCN和周围组织的时钟基因表达谱，并分析了SCN移植小鼠运动活动的昼夜节律。进行这些实验是为了阐明移植的SCN是否产生动态的昼夜节律振荡并保持可以在完整小鼠中检测到的相位关系。嫁接的SCN确实显示了时钟基因如mPeriod1（mPer1）和mPeriod2（mPer2）的动态昼夜节律表达节奏。此外，发现在移植的SCN中这些基因的表达节律与移植动物的运动活动节律之间的相位差与完整动物中的非常相似。此外，在移植动物的肝，肾和骨骼肌中，与完整动物一样，移植SCN的昼夜节律与周围组织的昼夜节律之间的相位角保持不变。但是，在SCN移植的动物中，mPer1和mPer2节律的振幅在周围组织中减弱。因此，我们目前的发现表明，移植的SCN具有产生动态内在的昼夜节律振荡的能力，并且还可以以与完整对照动物类似的方式锁定SCN，运动活动和周围振荡器之间的正常相角。

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