首页> 外文期刊>The East African medical journal >Arcobacter butzlieri strains from poultry abattoir effluent in Nigeria.
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Arcobacter butzlieri strains from poultry abattoir effluent in Nigeria.

机译:尼日利亚家禽屠宰场废水中的Butzlieri弧菌。

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OBJECTIVE: To investigate the prevalence, species distribution and genetic diversity of zoonotic Arcobacter species. DESIGN: Prospective study. SETTING: Drainage system of a cosmopolitan chicken abattoir in Lagos, Nigeria. METHODS: One hundred and fifty drainage water samples were enriched in a minimal antibiotics-containing medium at room temperature and bacteria then isolated by use of a membrane filtration method. RESULTS: Twenty six (14%) of samples were positive for Arcobacter spp. Of these, 20 were examined by a comprehensive probabilistic identification scheme for Epsilobacteria and all strains identified as A. butzleri. AFLP analysis of these strains revealed considerable genetic diversity among the strains, with 12 genotypes defined at the 90% similarity level. CONCLUSION: The prevalence of A. butzleri in Nigerian poultry abattoir effluent indicates this species may constitute a public health problem in this country. AFLP profiling could be a useful tool for molecular epidemiological and population genetic studies of this organism. This is the first known report of A. butzleri in Nigeria, and first application of AFLP analysis for genotyping the species.
机译:目的:探讨人畜共患弧菌的流行,种类分布及遗传多样性。设计:前瞻性研究。地点:尼日利亚拉各斯的一个国际性大屠宰场的排水系统。方法:在室温下,在最低限度的含抗生素培养基中富集150个排水样品,然后使用膜过滤法分离细菌。结果:26(14%)的样品为Arcobacter spp阳性。其中,通过全面的概率鉴定方案对20种细菌进行了检定,其中所有细菌均被鉴定为布氏芽孢杆菌。这些菌株的AFLP分析显示,这些菌株之间具有相当的遗传多样性,其中12个基因型的相似度定义为90%。结论:尼日利亚家禽屠宰场废水中普遍存在布氏曲霉,表明该物种可能构成该国的公共卫生问题。 AFLP分析可能是对该生物进行分子流行病学和群体遗传研究的有用工具。这是尼日利亚A. butzleri的第一个已知报道,也是AFLP分析在该物种的基因分型中的首次应用。

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