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首页> 外文期刊>The FEBS journal >A novel metallobridged bis(beta-cyclodextrin)s fluorescent probe for
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A novel metallobridged bis(beta-cyclodextrin)s fluorescent probe for

机译：一种新型的金属桥联双（β-环糊精）s荧光探针

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摘要

A novel metallobridged bis(beta-cyclodextrin)s 2 [bis(beta-CD)s 2] was synthesized and characterized by means of H-1 NMR, IR, element analysis and redox iodometric titration. The fluorescence of metallobridged bis(beta-CD)s 2 was weak compared with bis(beta-CD)s 1 because of the paramagnetism of copper (II) ions. Glutathione was able to form complexes with copper (II) derived from the metallobridged bis(beta-CD)s 2. This competitive complexation with copper (II) may lead to a significant fluorescence recovery of the bis(beta-CD)s. Therefore, a rapid and simple spectrofluorimetric method was developed for the determination of glutathione. The analytical application for glutathione was investigated in NaCl/P-i (pH 6.00) at room temperature. The linear range of the method was 0.30-20.0 mu mol.L-1 with a detection limit of 63.8 nmol.L-1. There was no interference from the plasma constituents. The proposed method had been successfully used to determine glutathione in human plasma.
机译：合成了新型的金属桥联的双（β-环糊精）2 [双（β-CD）2]，并通过H-1 NMR，IR，元素分析和氧化碘量滴定法进行了表征。金属桥联的双（β-CD）s 2的荧光比双（β-CD）s 1弱，这是因为铜（II）离子具有顺磁性。谷胱甘肽能够与衍生自金属桥联的双（β-CD）2的铜（II）形成络合物。与铜（II）的竞争性络合可能导致双（β-CD）的荧光显着恢复。因此，开发了一种快速简便的荧光光谱法测定谷胱甘肽。在室温下，在NaCl / P-i（pH 6.00）中研究了谷胱甘肽的分析应用。该方法的线性范围为0.30-20.0μmol.L-1，检测极限为63.8 nmol.L-1。没有血浆成分的干扰。所提出的方法已成功地用于测定人血浆中的谷胱甘肽。

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