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首页> 外文期刊>The FEBS journal >Pyrophosphate and tripolyphosphate affect firefly luciferase
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Pyrophosphate and tripolyphosphate affect firefly luciferase

机译：焦磷酸盐和三聚磷酸盐影响萤火虫荧光素酶

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摘要

The activating and stabilizing effects of inorganic pyrophosphate, tripolyphosphate and nucleoside triphosphates on firefly luciferase bioluminescence were studied. The results obtained show that those effects are a consequence of the luciferase-catalyzed splitting of dehydroluciferyl-adenylate, a powerful inhibitor formed as a side product in the course of the bioluminescence reaction. Inorganic pyrophosphate, tripolyphosphate, CTP and UTP antagonize the inhibitory effect of dehydroluciferyl-adenylate because they react with it giving rise to products that are, at least, less powerful inhibitors. Moreover, we demonstrate that the antagonizing effects depended on the rate of the splitting reactions being higher in the cases of inorganic pyrophosphate and tripolyphosphate and lower in the cases of CTP and UTP. In the case of inorganic pyrophosphate, the correlation between the rate of dehydroluciferyl-adenylate pyrophosphorolysis and the activating effect on bioluminescence only occurs for low concentrations because inorganic pyrophosphate is, simultaneously, an inhibitor of the bioluminescence reaction. Our results demonstrate that previous reports concerning the activating effects of several nucleotides (including some that do not react with dehydroluciferyl-adenylate) on bioluminescence were caused by the presence of inorganic pyrophosphate contamination in the preparations used.
机译：研究了无机焦磷酸盐，三聚磷酸盐和核苷三磷酸盐对萤火虫荧光素酶生物发光的激活和稳定作用。获得的结果表明，这些作用是萤光素酶催化的脱氢萤光素-腺苷酸分裂的结果，脱氢萤光素-腺苷酸是在生物发光反应过程中作为副产物形成的强大抑制剂。无机焦磷酸盐，三聚磷酸盐，CTP和UTP拮抗脱氢荧光素-腺苷酸的抑制作用，因为它们与之反应生成的产物至少是功能较弱的抑制剂。此外，我们证明了拮抗作用取决于拆分反应的速率，在无机焦磷酸盐和三聚磷酸盐的情况下较高，而在CTP和UTP的情况下较低。在无机焦磷酸盐的情况下，脱氢荧光素-腺苷酸焦磷酸解的速率与对生物发光的活化作用之间的相关性仅在低浓度下发生，因为无机焦磷酸盐同时是生物发光反应的抑制剂。我们的结果表明，以前有关某些核苷酸（包括不与脱氢荧光素-腺苷酸反应的核苷酸）对生物发光的激活作用的报道是由于所用制剂中存在无机焦磷酸盐污染引起的。

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