...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The FEBS journal >Metabolite profiling of plasma and urine from rats with TNBS-induced acute colitis using UPLC-ESI-QTOF-MS-based metabonomics - a pilot study
【24h】

Metabolite profiling of plasma and urine from rats with TNBS-induced acute colitis using UPLC-ESI-QTOF-MS-based metabonomics - a pilot study

机译：使用基于UPLC-ESI-QTOF-MS的代谢组学研究TNBS诱发的急性结肠炎大鼠血浆和尿液的代谢物谱-一项初步研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The incidence of inflammatory bowel disease, a relapsing intestinal condition whose precise etiology is still unclear, has continually increased over recent years. Metabolic profiling is an effective method with high sample throughput that can detect and identify potential biomarkers, and thus may be useful in investigating the pathogenesis of inflammatory bowel disease. In this study, using a metabonomics approach, a pilot study based on ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) was performed to characterize the metabolic profile of plasma and urine samples of rats with experimental colitis induced by 2,4,6-trinitrobenzene sulfonic acid. Acquired metabolic profile data were processed by multivariate data analysis for differentiation and screening of potential biomarkers. Five metabolites were identified in urine: two tryptophan metabolites [4-(2-aminophenyl)-2,4-dioxobutanoic acid and 4,6-cihydroxyquinoline], two gut microbial metabolites (phenyl-acetylglycine and p-cresol glucuronide), and the bile acid 12a-hydroxy-3-oxocholadienic acid. Seven metabolites were identified in plasma: three members of the bile acid/alcohol group (cholic acid, 12a-hydroxy-3-oxocholadienic acid and cholestane-3,7,12,24,25-pentol) and four lysophosphatidylcholines [LysoPC(20:4), LysoPC(16:0), LysoPC(18:1) and LysoPC(18:0)]. These metabolites are associated with damage of the intestinal barrier function, microbiota homeostasis, immune modulation and the inflammatory response, and play important roles in the pathogenesis of inflammatory bowel disease. Our results positively support application of the metabonomic approach in study of the pathophysiological mechanism of inflammatory bowel disease.
机译：炎症性肠病是一种复发性肠道疾病，其确切病因仍不清楚，近几年来一直在增加。代谢谱分析是一种具有高样品通量的有效方法，可以检测和鉴定潜在的生物标志物，因此在调查炎症性肠病的发病机理中可能有用。在这项研究中，使用代谢组学方法，基于超高效液相色谱结合电喷雾电离四极杆飞行时间质谱（UPLC-ESI-QTOF-MS）进行了一项初步研究，以表征血浆和血浆的代谢特征。 2,4,6-三硝基苯磺酸诱导的实验性结肠炎大鼠尿液样品。通过多变量数据分析处理获得的代谢谱数据，以区分和筛选潜在的生物标志物。尿液中鉴定出五种代谢物：两种色氨酸代谢物[4-（2-氨基苯基）-2,4-二氧代丁酸和4,6-环羟基喹啉]，两种肠道微生物代谢物（苯基-乙酰基甘氨酸和对甲酚葡糖醛酸）和胆汁酸12a-羟基-3-氧杂环丁二酸。在血浆中鉴定出7种代谢物：胆汁酸/酒精组的3个成员（胆酸，12a-羟基-3-氧杂环丁二酸和胆甾烷3,7,12,24,25-戊醇）和4个溶血磷脂酰胆碱[LysoPC（20 ：4），LysoPC（16：0），LysoPC（18：1）和LysoPC（18：0）]。这些代谢产物与肠屏障功能受损，微生物群稳态，免疫调节和炎性反应有关，并在炎性肠病的发病机理中起重要作用。我们的结果积极支持代谢组学方法在炎症性肠病病理生理机制研究中的应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号