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首页> 外文期刊>The Indian Journal of Animal Reproduction >EFFECT OF HUMAN ACTIVIN-A ON IN-VITRO MATURATION OF OOCYTES AND EARLY EMBRYONIC DEVELOPMENT IN BUFFALOES
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EFFECT OF HUMAN ACTIVIN-A ON IN-VITRO MATURATION OF OOCYTES AND EARLY EMBRYONIC DEVELOPMENT IN BUFFALOES

机译：人ACTIVIN-A对水牛卵母细胞体外成熟和早期胚胎发育的影响

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The present study was designed to assess the effect of human Activin-A on in-vitro maturation of oocytes and early embryonic development. The oocytes were subjected to in-vitro maturation for 22 h in Medium-199 supplemented with 10% serum, 0.5ug/ml FSH, 10 lU/ml LH, 1ug/ml Estradiol-17a, 20ng/ml EGF and 50uM Cysteamine in four groups i.e. Group-I (without Activin-A), Group-ll (Activin-A5 ng/ml), Group-Ill (Activin-A 10 ng/ ml), Group-IV (Activin-A20 ng/ml) in C02 incubator at 39°C temperature, 5% C02 and high humidity. The matured oocytes were co-incubated with 1x106 sperms of buffalo in modified synthetic oviductal fluid (mSOF) medium containing 10ug/ml heparin and after 22 h of sperm-oocyte incubation, fertilized oocytes were stripped off cumuluscells and cultured in mSOF medium for 7 days to study the embryonic development. A total of 665 oocytes were subjected to //7-raf/omaturation in four groups 165,167,166 and 167, respectively in groups I, II, III and IV and 148 (89.69%), 156 (93.41 %), 157 (94.57%) and 158 (94.61 %) oocytes were matured, respectively in groups I, II, III and IV with overall maturation rate of 93.08%. The cleavage rates were 39.86%, 48.07%, 53.50%, and 59.49%, respectively with overall cleavage rate of 50.40%. It was significantly (P<0.05) higher in group IV compared to group I and II (39.86 vs 48.07%). It was also significantly (P<0.05) higher in group III compared to group I (53.50 vs 39.07%). Embryonic development up to blastocyst stage was also significantly (P<0.05) higher in group IV as compared to group I and II (36.17 vs 16.95 and 21.33%). From the present study, it may be concluded that human Activin-A at 20ng/ml in maturation media significantly improved the cleavage rate and embryonic development up to blastocyst stage in buffalo.
机译：本研究旨在评估人激活素A对卵母细胞体外成熟和早期胚胎发育的影响。将卵母细胞在添加了10％血清，0.5ug / ml FSH，10 lU / ml LH，1ug / ml雌二醇17a，20ng / ml EGF和50uM半胱胺的培养基199中进行体外成熟22小时。 C02中的组，即组I（无激活素A），组II（激活素A5 ng / ml），组III（激活素A 10 ng / ml），组IV（激活素A20 ng / ml）在39°C温度，5％CO2和高湿度下进行培养。将成熟的卵母细胞与1x106的水牛精子在含有10ug / ml肝素的改良合成输卵管液（mSOF）培养基中共孵育，并在精子卵母细胞温育22小时后，剥离受精卵母细胞并在mSOF培养基中培养7天研究胚胎发育。第一，第二，第三和第四组的总共665个卵母细胞分别在165,167,166和167个四组中进行了// 7-raf /畸变，以及148（89.69％），156（93.41％），157（94.57％） I，II，III和IV组分别有158和94.61％的卵母细胞成熟，总成熟率为93.08％。卵裂率分别为39.86％，48.07％，53.50％和59.49％，总卵裂率为50.40％。 IV组比I组和II组显着（P <0.05）高（39.86 vs 48.07％）。与第一组相比，第三组也显着（P <0.05）高（53.50 vs 39.07％）。与I和II组相比，IV组直到胚泡期的胚胎发育也显着（P <0.05）高（36.17 vs 16.95和21.33％）。从本研究中可以得出结论，在成熟培养基中浓度为20ng / ml的人Activin-A可以显着改善水牛的卵母细胞的卵裂率和胚胎发育直至胚泡期。

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