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首页> 外文期刊>The international journal of developmental biology >Comparative expression analysis of Pax3 and Pax7 during mouse myogenesis
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Comparative expression analysis of Pax3 and Pax7 during mouse myogenesis

机译：Pax3和Pax7在小鼠成肌过程中的比较表达分析

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摘要

Pax3 and Pax7 are closely related transcription factors involved in the commitment of myogenic precursors in the developing trunk. However, it is not yet clear whether these genes are required for myogenic cell specification in the head and for post-somitic myogensis per se. In part, this uncertainty is due to the scarce information about their normal time course and pattern of expression. Here, we present a systematic immunohistochemical in situ analysis of spatio temporal characteristics of Pax3 and Pax7 protein expression in comparison to that of MyoD and myogenin in the developing trunk and head muscles. The observed patterns of expression suggest that Pax3 is not involved in myogenesis in the head and its post-somitic expression in the trunk and limb muscles is mostly repressed after stage E13.5. In contrast, Pax7 expression is shared among all striated muscles and exhibits a uniform pattern. Pax7 is expressed only in mononucleated cells that either differentiate into myotubes or later form satellite cells. During development of head muscles, expression of Pax7 follows expression of MyoD and myogenin, implying that Pax7 is not required to induce the initial steps of the myogenic program in the head. In Pax7 homozygous mutants, in which muscle development proceeds normally, expression of Pax3 is indistinguishable from its wild-type pattern (i.e. absent), suggesting that after stage E13.5 myogenesis does not require Pax3 and Pax7. These data challenge the concept that Pax3 and Pax7 determine a persistent lineage of myogenic precursors in pre-natal and post-natal muscle development.
机译：Pax3和Pax7是与发育中的躯干中成肌前体的参与密切相关的转录因子。然而，目前尚不清楚这些基因是否是头部的成肌细胞规格和自体体后成肌作用所必需的。这种不确定性部分是由于缺乏有关其正常时程和表达方式的信息。在这里，我们提出了系统的免疫组织化学原位分析时空特征的Pax3和Pax7蛋白表达与发展中的躯干和头部肌肉中的MyoD和Myogenin相比。观察到的表达模式表明，Pax3不参与头部的肌发生，在E13.5期后，其在躯干和四肢肌肉中的后声像表达主要受到抑制。相反，Pax7表达在所有横纹肌之间共享，并且表现出均匀的模式。 Pax7仅在分化为肌管或后来形成卫星细胞的单核细胞中表达。在头部肌肉发育过程中，Pax7的表达紧随MyoD和肌生成素的表达，这意味着不需要Pax7来诱导头部肌原性程序的初始步骤。在Pax7纯合突变体中，其中的肌肉发育正常进行，Pax3的表达与其野生型模式（即不存在）是无法区分的，这表明在E13.5阶段后，肌发生不需要Pax3和Pax7。这些数据挑战了Pax3和Pax7确定产前和产后肌肉发育中成肌前体的持久谱系的概念。

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