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首页> 外文期刊>The international journal of biochemistry and cell biology >Toxoplasma gondii grown in human cells uses GalNAc-containing glycosylphosphatidylinositol precursors to anchor surface antigens while the immunogenic Glc-GalNAc-containing precursors remain free at the parasite cell surface.
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Toxoplasma gondii grown in human cells uses GalNAc-containing glycosylphosphatidylinositol precursors to anchor surface antigens while the immunogenic Glc-GalNAc-containing precursors remain free at the parasite cell surface.

机译：在人类细胞中生长的弓形虫使用含GalNAc的糖基磷脂酰肌醇前体锚定表面抗原，而含免疫原性Glc-GalNAc的前体在寄生虫细胞表面保持游离。

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摘要

Toxoplasma gondii is a ubiquitous parasite that infects nearly all warm-blooded animals. Developmental switching in T. gondii, from the virulent tachyzoite to the relatively quiescent bradyzoite stage, is responsible for the disease propagation after alteration of the immune status of the carrier. The redifferentiation event is characterized by an over expression of a tachyzoite specific set of glycosylphosphatidylinositol anchored surface antigens and free GPIs. T. gondii grown in animal cells uses two glycosylphosphatidylinositol precursors to anchor the parasite surface proteins. The first form has an N-acetylgalactosamine residue bound to a conserved three-mannosyl core glycan, while the second structure contains an additional terminal glucose linked to the N-acetylgalactosamine side branch. Sera from persons infected with T. gondii reacted only with the glucose-N-acetylgalactosamine-containing structure. Here we report that T. gondii cultured in human cells uses predominantly the N-acetylgalactosamine-containing structure to anchor the parasite surface antigens. On the other hand, glycosylphosphatidylinositol structures having an additional terminal glucose are found exclusively on the parasite cell surface as free glycolipids participating in the production of cytokines that are implicated in the pathogenesis of T. gondii. We also provide evidence that such free glycosylphosphatidylinositols are restricted mainly to the lipid microdomains in the parasite cell surface membrane and mostly associated with proteins involved in the parasite motility as well as invasion of the host cell.
机译：弓形虫是一种普遍存在的寄生虫，几乎感染了所有温血动物。刚地弓形虫从有毒的速殖子到相对静止的缓殖子阶段的发育转换，是导致载体免疫状态改变后疾病传播的原因。重分化事件的特征是糖基磷脂酰肌醇锚定的表面抗原和游离GPI的速殖子特异性组的过表达。在动物细胞中生长的弓形虫使用两种糖基磷脂酰肌醇前体来锚定寄生虫表面蛋白。第一种形式具有结合至保守的三甘露糖基核心聚糖上的N-乙酰半乳糖胺残基，而第二种结构包含连接至N-乙酰半乳糖胺侧分支的另外的末端葡萄糖。刚地弓形虫感染者的血清仅与含葡萄糖-N-乙酰半乳糖胺的结构反应。在这里，我们报告在人类细胞中培养的弓形虫主要使用含N-乙酰半乳糖胺的结构锚定寄生虫表面抗原。另一方面，仅在寄生虫细胞表面上发现了具有额外末端葡萄糖的糖基磷脂酰肌醇结构，其为游离糖脂，参与了与弓形虫的发病有关的细胞因子的产生。我们还提供证据表明这种游离的糖基磷脂酰肌醇主要限于寄生虫细胞表面膜中的脂质微区，并且主要与参与寄生虫运动性和宿主细胞入侵的蛋白质有关。

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