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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Immunocytochemical evidence of calreticulin-like protein in pollen tubes and styles of Petunia hybrida Hort.
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Immunocytochemical evidence of calreticulin-like protein in pollen tubes and styles of Petunia hybrida Hort.

机译：花粉管中钙网蛋白样蛋白的免疫细胞化学证据和矮牵牛花的样式。

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摘要

With a polyclonal antibody raised against calreticulin (CRT) the locations where the protein occurs in unpollinated and pollinated styles of Petunia hybrida were localized. The epitopes binding the CRT antibody were immunolocalized preferentially in pollen tubes. In transmitting tract cells, both before and after pollination, the level of CRT was low. The protein was mainly localized in the cytosol and around dictyosomes of transmitting-tract cells. In pollen tubes, a high level of CRT was found at their tips rich in endoplasmatic reticulum, cisternae piles of reticular and/or dictyosomal origin, and vesicles. Binding sites of the CRT antibody were also found in the internal callosic cell wall of the pollen tube, These results indicate a role of CRT in cells directly participating in pollen-pistil interaction. [References: 77]
机译：使用针对钙网蛋白（CRT）的多克隆抗体，可以确定该蛋白以未授粉和授粉形式的矮牵牛的发生位置。结合CRT抗体的表位优先在花粉管中进行免疫定位。在传粉之前和之后的传播道细胞中，CRT的水平都很低。该蛋白主要位于胞质溶胶和传输道细胞的双体周围。在花粉管中，发现在其尖端有大量的CRT，这些CRT富含内质网，网状和/或双体来源的水箱堆以及囊泡。在花粉管的内部愈伤组织细胞壁中也发现了CRT抗体的结合位点。这些结果表明CRT在直接参与花粉-雌蕊相互作用的细胞中的作用。 [参考：77]

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