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首页> 外文期刊>Protist >Association of fucoxanthin chlorophyll a/c-binding polypeptides with photosystems and phosphorylation in the centric diatom Cyclotella cryptica
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Association of fucoxanthin chlorophyll a/c-binding polypeptides with photosystems and phosphorylation in the centric diatom Cyclotella cryptica

机译：岩藻黄质叶绿素a / c结合多肽与光系统和中枢硅藻密闭小环藻磷酸化的关联

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Solubilization of thylakoid membranes of Cyclotella cryptica with dodecyl-beta maltoside followed by sucrose density gradient centrifugation or deriphate polyacrylamide gel electrophoresis resulted in the isolation of pigment protein complexes. These complexes were characterized by absorption and fluorescence spectroscopy, sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western immunoblotting using antisera against fucoxanthin chlorophyll a/c-binding proteins and the reaction center protein D2 of photosystem II. Sucrose density gradient centrifugation yielded four bands. Band 1 consisted of free pigments with minor amounts of fucoxanthin chlorophyll a/c-binding proteins. Bands 2, 3, and 4 represented a major fucoxanthin chlorophyll a/c-binding protein fraction, photosystem II, and photosystem 1, respectively. Deriphate polyacrylamide gel electrophoresis gave rise to five bands, representing photosystem 1, photosystem II, two fucoxanthin chlorophyll a/c-binding protein complexes, and a band mostly consisting of free pigments. In the Western immunoblotting experiments, the specific association of two fucoxanthin chlorophyll a/c-binding proteins, Fcp2 and Fcp4, to the photosystems could be demonstrated. In vivo experiments using antibodies against phosphothreonine residues and in vitro studies using [gamma-P-32]ATP showed that fucoxanthin chlorophyll a/c binding-proteins of 22 kDa became phosphorylated. (c) 2006 Elsevier GmbH. All rights reserved.
机译：用十二烷基-β-麦芽糖苷增溶神秘小环藻类囊体膜，然后进行蔗糖密度梯度离心或去离子聚丙烯酰胺凝胶电泳，分离出色素蛋白复合物。这些复合物的特征在于吸收和荧光光谱，十二烷基硫酸钠聚丙烯酰胺凝胶电泳和使用针对岩藻黄质叶绿素a / c结合蛋白和光系统II的反应中心蛋白D2的抗血清进行的Western免疫印迹。蔗糖密度梯度离心产生四个谱带。条带1由游离色素和少量的黄藻黄质叶绿素a / c结合蛋白组成。条带2、3和4分别代表了主要的岩藻黄质叶绿素a / c结合蛋白部分，光系统II和光系统1。脱氟聚丙烯酰胺凝胶电泳产生了五个带，分别代表光系统1，光系统II，两个岩藻黄质叶绿素a / c结合蛋白复合物，以及一个主要由游离色素组成的带。在Western免疫印迹实验中，可以证明两种岩藻黄质叶绿素a / c结合蛋白Fcp2和Fcp4与光系统的特异性联系。使用抗磷酸苏氨酸残基的抗体进行的体内实验和使用[γ-P-32] ATP的体外研究表明，22 kDa的岩藻黄质叶绿素a / c结合蛋白被磷酸化了。 （c）2006 Elsevier GmbH。版权所有。

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