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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Immunolocalisation of arabinogalactan proteins and pectins in Actinidia deliciosa pollen
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Immunolocalisation of arabinogalactan proteins and pectins in Actinidia deliciosa pollen

机译：阿拉伯半乳聚糖蛋白和果胶在猕猴桃花粉中的免疫定位

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摘要

The cell wall composition of germinating pollen grains of Actinidia deliciosa was studied by immunolocalization with monoclonal antibodies against arabinogalactan proteins (AGPs) and pectins. In ungerminated pollen, the JIM8 epitope (against a subset of AGPs) was located in the intine and in the cytoplasm, while the MAC207 epitope (against AGPs) was located only in the exine. After germination, the JIM8 and MAC 207 epitopes were located in the cytoplasm and in the pollen tube wall. The Yariv reagent that binds to AGPs was added to the germination medium inducing a reduction or inhibition in pollen germination. This indicates that AGPs are present in the growing pollen tube and play an important role in pollen germination. To identify the nature of the pectins found in pollen grains and tubes, four monoclonal antibodies were used. The JIM5 epitope (against unesterified pectins) was located in the intine, more intensely in the pore region, and along the pollen tube wall, and the JIM7 epitope (against methylesterified pectins) was also observed in the cytoplasm. After germination, the JIM5 epitope was located in the pollen tube wall; although, the tube tip was not labelled. The JIM7 epitope was located in the entire pollen tube wall. LM5 (against galactans) showed a labelling pattern similar to that of JIM5 and the pattern of LM6 (against arabinans) was similar to that of JIM7. Pectins show different distribution patterns when the degree of esterification is considered. Pollen tube wall pectins are less esterified than those of the pollen tube tip. The association of AGPs with pectins in the cell wall of the pollen grain and the pollen tube may play an important role in the maintenance of cell shape during pollen growth and development.
机译：通过用抗阿拉伯半乳聚糖蛋白（AGPs）和果胶的单克隆抗体免疫定位研究了猕猴桃的发芽花粉粒的细胞壁组成。在未发芽的花粉中，JIM8表位（针对AGP的一个子集）位于大肠菌丝和细胞质中，而MAC207表位（针对AGP）仅位于外在的花粉中。发芽后，JIM8和MAC 207表位位于细胞质和花粉管壁中。将与AGP结合的Yariv试剂添加到发芽培养基中，以诱导花粉萌发的减少或抑制。这表明AGP存在于正在生长的花粉管中，并在花粉萌发中起重要作用。为了鉴定在花粉粒和管中发现的果胶的性质，使用了四种单克隆抗体。 JIM5表位（针对未酯化的果胶）位于花粉中，更紧密地位于孔区域中，沿着花粉管壁，在细胞质中也观察到JIM7表位（针对甲酯化的果胶）。发芽后，JIM5表位位于花粉管壁中。虽然，管尖未标记。 JIM7表位位于整个花粉管壁中。 LM5（针对半乳聚糖）显示出与JIM5相似的标记模式，而LM6（针对阿拉伯聚糖）与JIM7相似。当考虑酯化程度时，果胶显示出不同的分布模式。花粉管壁果胶的酯化程度低于花粉管头的果胶。在花粉生长和发育过程中，AGP与花粉粒和花粉管细胞壁中果胶的结合可能在维持细胞形状中起重要作用。

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