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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Organelle acidification is important for localisation of vacuolar proteins in Saccharomyces cerevisiae
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Organelle acidification is important for localisation of vacuolar proteins in Saccharomyces cerevisiae

机译：细胞器酸化对于酿酒酵母中液泡蛋白的定位很重要

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摘要

The acidic environments in the vacuole and other acidic organelles are important for many cellular processes in eukaryotic cells. In this study, we comprehensively investigated the roles of organelle acidification in vacuolar protein localisation in Saccharomyces cerevisiae. After repressing the acidification of acidic compartments by treatment with concanamycin A, a specific inhibitor of vacuolar H+-ATPase (V-ATPase), we examined the localisation of GFP-fused proteins that were predicted to localise in the vacuolar lumen or on the vacuolar membrane. Of the 73 proteins examined, 19 changed their localisation to the cytoplasmic region. Localisation changes were evaluated quantitatively using the image processing programme CalMorph. The delocalised proteins included vacuolar hydrolases, V-ATPase subunits, transporters and enzymes for membrane biogenesis, as well as proteins required for protein transport. These results suggest that many alterations in the localisation of vacuolar proteins occur after loss of the acidification of acidic compartments.
机译：液泡和其他酸性细胞器中的酸性环境对于真核细胞中的许多细胞过程都很重要。在这项研究中，我们全面研究了细胞器酸化在酿酒酵母液泡蛋白定位中的作用。通过用刀豆球蛋白H + -ATPase（V-ATPase）的特异性抑制剂伴刀豆球蛋白A抑制酸性区室的酸化后，我们检查了GFP融合蛋白的定位，该蛋白预计位于液泡腔或液泡膜中。 。在检查的73种蛋白质中，有19种改变了它们的定位至胞质区域。使用图像处理程序CalMorph定量评估定位变化。离域的蛋白质包括液泡水解酶，V-ATPase亚基，膜生物发生的转运蛋白和酶，以及蛋白质转运所需的蛋白质。这些结果表明，在失去酸性区室的酸化之后，液泡蛋白的定位发生了许多改变。

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