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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >CHLOROPLAST ASSEMBLAGE BY MECHANICAL STIMULATION AND ITS INTERCELLULAR TRANSMISSION IN DIATOM CELLS
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CHLOROPLAST ASSEMBLAGE BY MECHANICAL STIMULATION AND ITS INTERCELLULAR TRANSMISSION IN DIATOM CELLS

机译：机械刺激的叶绿体组装及其在间质细胞中的细胞间传递

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By touching a cell of Pleurosira laevis, a centric diatom, the chloroplasts in the cortical cytoplasm immediately migrate to the nuclear cytoplasm which is located at the center of the cell. The time required for migration, is about 10 s and that for restoration is about 15 min. The touch could be replaced by an electric stimulation. A 7 V pulse for 1 ms was required for the induction of chloroplast assemblage. To obtain chloroplast assemblage in 50% of the cells, the amount of time and voltage required were inversely proportional. Higher concentrations of KCl also induced chloroplast assemblage. Neither contact nor electric stimulation in Ca2+ free medium induced chloroplast assemblage. Verapamil, a Ca2+ channel blocker, in medium suppressed the chloroplast assemblage. Ca2+ ionophore caused the chloroplast assemblage. These results suggested that the depolarization of the plasma membrane was a trigger of the induction of chloroplast assemblage and the influx of Ca2+ to the cytoplasm through the channel was essential for this. The chloroplast assemblage was transmitted successively to the other cells of the filamentous colony, though these cells were linked only by a mucilaginous substance. Furthermore, the transmission occurred in cells which were placed separately. The maximum distance possible for this jumping transmission was 200 mu m in the culture medium. To search for the release of any chemical transmitter, contact stimulation was applied to one cell at the middle of a filamentous colony which was placed in water streaming along the axis of the filament. Chloroplast assemblage was induced more in downstream cells than in upstream ones. This result suggested the existence of some substance which mediated the transmission of the stimulation. [References: 21]
机译：通过接触一个中心硅藻Pleurosira laevis的细胞，皮层细胞质中的叶绿体立即迁移到位于细胞中心的核细胞质。迁移所需的时间约为10 s，恢复所需的时间约为15分钟。可以用电刺激代替触摸。诱导叶绿体组装需要7 V脉冲持续1 ms。为了在50％的电池中获得叶绿体组装体，所需的时间和电压成反比。较高浓度的KCl也会诱导叶绿体聚集。不含Ca2 +的培养基中的接触和电刺激均不会诱导叶绿体组装。维拉帕米，一种Ca2 +通道阻滞剂，在培养基中抑制了叶绿体的组装。 Ca 2+离子载体引起叶绿体组装。这些结果表明，质膜的去极化是诱导叶绿体组装的触发因素，而Ca2 +通过通道向细胞质的流入是必不可少的。尽管这些细胞仅通过粘液性物质连接，但叶绿体的组装顺序地传递至丝状菌落的其他细胞。此外，传播发生在分开放置的细胞中。在培养基中，这种跳跃传播的最大可能距离为200微米。为了寻找任何化学递质的释放，将接触刺激施加于丝状菌落中间的一个细胞，该细胞置于沿着丝状轴流动的水中。叶绿体组装在下游细胞中比在上游细胞中诱导更多。该结果表明介导刺激传播的某种物质的存在。 [参考：21]

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