...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >The cantharidin-induced oxidative burst in tobacco BY-2 cell suspension cultures
【24h】

The cantharidin-induced oxidative burst in tobacco BY-2 cell suspension cultures

机译：烟碱素诱导烟草BY-2细胞悬浮培养物中的氧化爆发

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The in vivo induction of H2O2 production was tested on tobacco cell suspension cultures (Nicotiana tabacum cv. Bright Yellow-a). The measurement of H2O2 was based on the oxidation of 3,5- dichloro-2-hydroxybenzensulfonic acid by endogenous peroxidases and spectrophotometric detection after reaction with 4-aminoantipyrine. The phosphatase inhibitor cantharidin induced a transient increase in H2O2 synthesis. The timing of the H2O2 production, the level of induction by cantharidin and the background H2O2 production were dependent on the tobacco cell concentration used. A concentration curve of cantharidin revealed saturating kinetics for the H2O2 detection (E-50 = 46 to 70 mu M, E-max = 101 to 128 mu mol/h . g fresh weight). An inhibitor study with the tobacco BY-2 cells showed high inhibitions of the H2O2 induction with the flavin analogues diphenylene iodonium (I-50 = 1.26 mu M) and acridine orange and with membrane-permeative thiol reagents (N-ethyl maleimide, N-pyrene maleimide, iodoacetate); whereas the nonpermeative thiol reagent p-chloromercuribenzoic acid was ineffective. Therefore, the induction of H2O2 production with phosphatase inhibitors (cantharidin) showed comparable properties to the elicitor-induced oxidative-burst response in other plant cells. [References: 37]
机译：在烟草细胞悬浮培养物（Nicotiana tabacum cv。Bright Yellow-a）上测试了体内H2O2的诱导。 H2O2的测定基于内源性过氧化物酶氧化3,5-二氯-2-羟基苯磺酸和与4-氨基安替比林反应后的分光光度法。磷酸酶抑制剂cantharidin引起H2O2合成的瞬时增加。 H2O2产生的时机，斑th素的诱导水平和背景H2O2产生取决于所用烟草细胞的浓度。斑th素的浓度曲线揭示出用于H 2 O 2检测的饱和动力学（E-50 ＝ 46至70μM，E-max ＝ 101至128μmol/ h.g鲜重）。用烟草BY-2细胞进行的抑制剂研究表明，黄素类似物二亚苯基碘鎓（I-50 = 1.26μM）和a啶橙以及膜渗透性硫醇试剂（N-乙基马来酰亚胺，N- male马来酰亚胺，碘乙酸盐）;非渗透性硫醇试剂对氯mercuribenzoic酸无效。因此，用磷酸酶抑制剂（cantharidin）诱导H2O2的产生与诱导物诱导的其他植物细胞的氧化爆发反应具有可比的特性。 [参考：37]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号