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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Inhibition of beta-1,3-Glucan Synthase and Cell Growth of Cryptococcus species by Recombinant Single-chain Anti-i-iotypic Antibodies
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Inhibition of beta-1,3-Glucan Synthase and Cell Growth of Cryptococcus species by Recombinant Single-chain Anti-i-iotypic Antibodies

机译：重组单链抗i型抗体对β-1,3-葡聚糖合酶的抑制和隐球菌物种的细胞生长

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摘要

Recombinant single-chain fragment variable(scFv)anti-idiotypic antibodies were produced to represent the internal image of a HM-1 killer toxin,which is characterized by a wide spectrum of anti-fungal activity through inhibiting beta-1,3-glucan synthase(GS).We examined if scFv antibodies are active against Cryptococcus species,a human pathogen of increasing medical importance.The anti-cryptococcal activity of scFv antibodies and HM-1 were assessed by MIC analysis for C.neoformans IFM 40215 and C.albidus NBRC 0612 cells.The scFv antibodies had strong anti-cryptococcal activity in vitro with IC_(50)at 1.07X10~(-7)to 2.60X10~(-7)M for C.neoformans and C.albidus.Furthermore,the scFv antibodies potentially inhibited GS of C.neoformans with IC_(50)at I.27XI0~(-7)to 2.27X10~(-7)M.Both the anti-fungal and anti-GS activities of the scFv antibodies were markedly neutralized by the monoclonal antibody that neutralizes HM-1 killer toxin.
机译：产生了重组单链片段可变（scFv）抗独特型抗体，以代表HM-1杀手毒素的内部图像，其特征在于通过抑制β-1,3-葡聚糖合酶具有广泛的抗真菌活性（GS）。我们检查了scFv抗体是否对具有日益重要医学意义的人类病原体隐球菌具有活性。通过MIC分析评估了新孢菌IFM 40215和C.albidus的scFv抗体和HM-1的抗隐球菌活性。 NBRC 0612细胞.scFv抗体具有很强的抗隐球菌活性，IC_（50）在1.07X10〜（-7）至2.60X10〜（-7）M时对新福尔摩斯氏杆菌和C.albidus有抑制作用。抗体可能会抑制I.27XI0〜（-7）处IC_（50）至2.27X10〜（-7）M的新孢子虫的GS.scFv抗体的抗真菌和抗GS活性均被显着中和中和HM-1杀伤毒素的单克隆抗体。

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