Identification of L-Glutamine :2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in bacillus circulans

Hideyuki Tamegai; Eriko Nango; Mieko Kuwahara; Katsumi Kakinuma

首页> 外文期刊>The Journal of Antibiotics: An International Journal >Identification of L-Glutamine :2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in bacillus circulans

【24h】

Identification of L-Glutamine :2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in bacillus circulans

机译：细菌丁环素生物合成中所需的L-谷氨酰胺：2-脱氧-鞘氨醇氨基转移酶的鉴定

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Using inverse PCR,two new genes (btrN and btrS) were identified upstream of the putative glycosyltransferase gene btrM in the butirosin-biosynthetic btr gene cluster of Bacillus circulans.The upstream gene btrS showed significant homology with stsC of Streptomyces griseus,which encodes L-glutamine:scyllo-inosose aminotransferase in the biosynthesis of streptomycin.The function of BtrS was further confirmed by heterologous expression in Escherichia coli and chemical identification of the conversion of 2-deoxy-scylloinosose into 2-deoxy-scyllo-inosamine.The identification of BtrS as L-glutamine:2-deoxy-scryllo-inosose aminotransferase is the first report of the aminotransferase gene responsible for 2-deoxystreptamine biosynthesis.

机译：通过反向PCR，在环孢芽孢杆菌丁酸生物合成btr基因簇的推定糖基转移酶基因btrM的上游发现了两个新基因（btrN和btrS）。上游基因btrS与灰色链霉菌的stsC具有显着的同源性，其编码L谷氨酰胺：芥子油苷氨基转移酶在链霉素的生物合成中.BtrS的功能通过在大肠杆菌中异源表达以及从2-脱氧芥子糖转化为2-脱氧芥子糖胺的化学鉴定得到了进一步的证实。作为L-谷氨酰胺：2-脱氧-鞘氨糖基氨基转移酶，是负责2-脱氧链胺生物合成的氨基转移酶基因的首次报道。

著录项

来源
《The Journal of Antibiotics: An International Journal》 |2002年第8期|共8页
作者
Hideyuki Tamegai; Eriko Nango; Mieko Kuwahara; Katsumi Kakinuma;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类药学;药品;
关键词

相似文献

外文文献
中文文献
专利

1. Identification of L-Glutamine :2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in bacillus circulans [J] . Hideyuki Tamegai, Eriko Nango, Mieko Kuwahara, The Journal of Antibiotics: An International Journal . 2002,第8期

机译：细菌丁环素生物合成中所需的L-谷氨酰胺：2-脱氧-鞘氨醇氨基转移酶的鉴定
2. Characterization of the Enzyme BtrD from Bacillus circulans and Revision of Its Functional Assignment in the Biosynthesis of Butirosin [J] . Andrew W. Truman, Fanglu Huang, Nicholas M. Llewellyn, Angewandte Chemie . 2007,第9期

机译：圆环芽孢杆菌酶BtrD的表征及其在布丁素生物合成中的功能分配
3. Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium [J] . A. Ian SCOTT, Alain RAMBACH, Charles A. ROESSNER, The biochemical journal . 2003,第2期

机译：巨大芽孢杆菌中西罗海姆和钴胺素生物合成中precorrin-2脱氢和金属离子插入所需酶的鉴定和功能分析
4. Biosorption of Bacillus circulans and Bacillus mucilaginosus on the Surface of Diaspore, Kaolinite and Quartz [C] . SUN Desi, Bao Hong, CHEN Hao, International conference on biotechnology, chemical and materials engineering . 2012

机译：游动芽孢杆菌，粘液芽孢杆菌在渗水孔，高岭土和石英表面的生物吸附
5. Study of a Bacillus circulans chitin -binding domain by a green fluorescent protein binding assay and detection of lysozymes by improved zymograms. [D] . Hardt, Markus. 2002

机译：通过绿色荧光蛋白结合测定法研究环生芽孢杆菌的几丁质结合结构域，并通过改进的酶谱图检测溶菌酶。
6. Relationship between butirosin biosynthesis and sporulation in Bacillus circulans. [O] . D H Nam, D D Ryu 1985

机译：丁酸芽孢杆菌生物合成与芽孢杆菌循环中的孢子形成之间的关系。
7. Biochemical Studies on 2-deoxy-scyllo-inosose, an early intermediate in biosynthesis of 2-dexystreptamin. Part VI. Kinetic Isotope Effect and Reaction Mechanism of 2-Deoxy-scyllo-inosose Synthase Derived from Butirosin-producing Bacillus circulans. [O] . FUMITAKA KUDO, NORIAKI YAMAUCHI, RIEKO SUZUKI, 1997

机译：2-脱氧 - Scyllo-inososose的生化研究，2-Dexystreptamin的生物合成早期中间体。第VI部分。豆类素芽孢杆菌芽孢杆菌芽孢杆菌芽孢杆菌芽孢杆菌的2-脱氧 - Scyllo-Inosose合酶的动力学同位素效应与反应机理。
8. Characterisation of Potential Antimicrobial Targets in Bacillus spp. I. Aminotransferases and Methionine Regeneration in Bacillus subtilis. [R] . Berger, B. J., Knodel, M. H. 2002

机译：芽孢杆菌潜在抗菌靶标的表征I.枯草芽孢杆菌中的氨基转移酶和蛋氨酸再生。

Identification of L-Glutamine :2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in bacillus circulans

摘要

著录项

相似文献

相关主题

期刊订阅