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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Complex formation between primycin and ergosterol: Entropy-driven initiation of modification of the fungal plasma membrane structure
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Complex formation between primycin and ergosterol: Entropy-driven initiation of modification of the fungal plasma membrane structure

机译：伯霉素和麦角固醇之间的复合物形成：真菌驱动的质膜结构修饰的熵驱动引发

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摘要

The interaction of the antibiotic primycin with the main fungal sterol, ergosterol, was investigated in vitro in order to monitor the effect of primycin on the fungal plasma membrane at the molecular level. The thermodynamic parameters of complex formation were determined by measuring Rayleigh scattering as a signal sensitive to particle size. The Benesi-Hildebrand method validated the 1: 1 stoichiometry of the primycin-ergosterol complexes. A very low enthalpy change (ΔH=1.14 kJ mol -1) was measured during the complex formation, which itself cannot be responsible for the molecular association. However, the entropy production (ΔS=29.78 J mol K -1) observed during the complex formation can describe the molecular interaction. This effect is probably due to the partial destruction of the solvation shell of the interacting species before the interlinking of the molecules. The results highlight the importance of ergosterol as concerns the mode of effect of primycin in the treatment of fungal infections. As the entropy has a determinant role in the ergosterol-primycin interaction, this interaction exhibits a very high temperature dependence, with the important consequence that the effect exerted by primycin on the cell membranes increases with rising temperature, and the effect is therefore pronounced in fevered bodies.
机译：体外研究了抗生素伯霉素与主要真菌固醇麦角固醇的相互作用，以便在分子水平上监测伯霉素对真菌质膜的影响。通过测量瑞利散射作为对粒度敏感的信号来确定复合物形成的热力学参数。 Benesi-Hildebrand方法验证了伯霉素-麦角固醇复合物的化学计量比为1：1。在络合物形成过程中测得非常低的焓变（ΔH= 1.14 kJ mol -1），这本身不能引起分子缔合。然而，在复合物形成期间观察到的熵产生（ΔS＝ 29.78J mol K -1）可以描述分子相互作用。该作用可能是由于在分子交联之前相互作用物种的溶剂化壳的部分破坏。结果突出了麦角固醇的重要性，涉及到伯霉素在真菌感染治疗中的作用方式。由于熵在麦角固醇-伯霉素的相互作用中起决定性作用，因此这种相互作用表现出非常高的温度依赖性，其重要的结果是，伯霉素对细胞膜的作用随温度升高而增加，因此在发烧时这种作用尤为明显。身体。

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