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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Effect of a novel antibiotic, heliquinomycin, on DNA helicase and cell growth
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Effect of a novel antibiotic, heliquinomycin, on DNA helicase and cell growth

机译：新型抗生素Heliquinomycin对DNA解旋酶和细胞生长的影响

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摘要

Heliquinomycin a novel microbial product, was found to inhibit a human DNA helicase enzyme isolated from HeLa S3 cells at concentrations of 5 to 10 mu g/ml. In contrast, adriamycin, etoposide and cisplatin did not inhibit this enzyme at the concentrations tested. Furthermore, the replication and repair of SV40 chromosome were not affected at heliquinomycin concentration of 50 mu g/ml. The topoisomerase II and I enzymes were inhibited at 30 mu g/ml and 100 mu g/ml of heliquinomycin, respectively. Heliquinomycin inhibited the growth of HeLa S3, KB, LS180, K562 and HL60 human tumor cell lines at IC50 values of 0.96 to 2.8 mu g/ml. In addition, the growth of adriamycin and cisplatin resistant P388 cell lines were inhibited at similar concentrations. Heliquinomycin inhibited both DNA and RNA synthesis in cell culture but did not inhibit protein synthesis. HeLa S3 cells were arrested at the G(2)/M phase by heliquinomycin. These studies suggest that heliquinomycin is a selective inhibitor of a cellular DNA helicase and in turn, inhibits growth of tumor cell lines. [References: 32]
机译：发现Helinomynomycin是一种新型的微生物产品，其以5至10μg / ml的浓度抑制从HeLa S3细胞分离的人DNA解旋酶。相反，在所测试的浓度下，阿霉素，依托泊苷和顺铂不抑制该酶。此外，在50μg/ ml的螺旋霉素浓度下，SV40染色体的复制和修复不受影响。拓扑异构酶II和I酶分别被Heliquinomycin抑制为30μg / ml和100μg / ml。 Heliquinomycin抑制HeLa S3，KB，LS180，K562和HL60人肿瘤细胞系的生长，IC50值为0.96至2.8μg / ml。另外，在相似的浓度下，阿霉素和顺铂抗性P388细胞系的生长受到抑制。螺旋霉素同时抑制细胞培养中的DNA和RNA合成，但不抑制蛋白质合成。 HeLa S3细胞被Heliquinomycin阻滞在G（2）/ M期。这些研究表明，螺旋霉素是细胞DNA解旋酶的选择性抑制剂，进而抑制肿瘤细胞系的生长。 [参考：32]

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