...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of molecular diagnostics: JMD >Identification of the tumor cells in peripheral T-cell lymphomas by combined polymerase chain reaction-based T-cell receptor beta spectrotyping and immunohistological detection with T-cell receptor beta chain variable region segment-specific antibodi
【24h】

Identification of the tumor cells in peripheral T-cell lymphomas by combined polymerase chain reaction-based T-cell receptor beta spectrotyping and immunohistological detection with T-cell receptor beta chain variable region segment-specific antibodi

机译：通过基于聚合酶链反应的T细胞受体β光谱分析和T细胞受体β链可变区节段特异性抗体的免疫组织学检测，鉴定外周T细胞淋巴瘤中的肿瘤细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Most nodal peripheral T-cell lymphomas (PTCL) originate from alphabeta-T cells, and they often contain reactive T cells that may hamper immunophenotyping. To specifically identify the neoplastic population in immunohistochemically stained slides, we assessed the heterogeneity of the T-cell receptor beta chain variable region (TCRVbeta). This region contains 65 gene segments, of which only one is expressed after rearrangement. To investigate PTCL, we developed a polymerase chain reaction assay to define the clonally rearranged TCRVbeta segment. Detecting the corresponding epitope with segment-specific antibodies enabled identification of tumor cells among the T cells. The TCRVbeta segment of the tumor cells was defined in 13 of 13 PTCL not otherwise specified and 11 of 13 angioimmunoblastic T-cell lymphomas. Antibodies corresponding to the respective TCRVbeta segment of the tumor were available for seven cases from each group. After applying these antibodies in combination with antibodies against CD3, CD5, CD4, CD8, and cytotoxic molecules, double stains were evaluated by confocal laser scanning microscopy. In 9 of 14 cases, less than 50% of T cells expressed the clonally rearranged TCRVbeta segment. Phenotypes defined in double stains differed from those obtained by conventional immunohistochemistry in 11 of 14 cases. The combination of TCRVbeta polymerase chain reaction and immunohistochemistry may facilitate more reliable detection and characterization of tumor cells in PTCL.
机译：大多数淋巴结周围性T细胞淋巴瘤（PTCL）起源于字母T细胞，并且它们通常含有反应性T细胞，可能会妨碍免疫表型。为了在免疫组织化学染色的载玻片上特异性鉴定赘生物，我们评估了T细胞受体β链可变区（TCRVbeta）的异质性。该区域包含65个基因片段，其中只有一个在重排后表达。为了研究PTCL，我们开发了一种聚合酶链反应测定法来定义克隆重排的TCRVbeta段。用区段特异性抗体检测相应的表位使得能够鉴定T细胞中的肿瘤细胞。肿瘤细胞的TCRVbeta节段在未另外说明的13个PTCL中的13个和13个血管免疫母细胞性T细胞淋巴瘤中的11个中定义。每组有7例可获得与肿瘤各自TCRVbeta片段相对应的抗体。将这些抗体与针对CD3，CD5，CD4，CD8和细胞毒性分子的抗体联合使用后，通过共聚焦激光扫描显微镜评估了双色斑。在14例中的9例中，不到50％的T细胞表达了克隆重排的TCRVbeta片段。在14例病例中有11例在双重染色中定义的表型与通过常规免疫组织化学获得的表型不同。 TCRVbeta聚合酶链反应和免疫组织化学的结合可以促进PTCL中肿瘤细胞更可靠的检测和表征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号