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首页> 外文期刊>Chromatographia >SIMULTANEOUS DETERMINATION OF LOSARTAN AND ACTIVE METABOLITE EXP3174 IN RAT PLASMA BY HPLC WITH COLUMN SWITCHING
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SIMULTANEOUS DETERMINATION OF LOSARTAN AND ACTIVE METABOLITE EXP3174 IN RAT PLASMA BY HPLC WITH COLUMN SWITCHING

机译：色谱柱切换同时测定大鼠血浆中的洛沙坦和活性代谢物EXP3174

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摘要

A new liquid chromatographic method with column-switching has been developed for the simultaneous determination of losartan and its active metabolite, EXP3174 in rat plasma. The plasma sample was injected onto a precolumn of LiChroprep RP-8 after dilution with 5% acetonitrile in 50 mM phosphoric acid. Polar plasma components were eluted using this diluent. After valve switching, the concentrated drugs were eluted in the back-flush mode and separated by an Inertsil ODS-2 column with acetonitrile-acetate buffer. The method showed excellent precision, accuracy and speed with detection limit 20 ng mL(-1). Total analysis time per sample was less than 40 min and the coefficients of variation for intra and inter-assay were 4.8%. This method has been successfully applied after oral administration of losartan to rat plasma samples. [References: 19]
机译：开发了一种具有柱切换功能的液相色谱新方法，用于同时测定大鼠血浆中的氯沙坦及其活性代谢物EXP3174。将血浆样品在50 mM磷酸中用5％乙腈稀释后，注入LiChroprep RP-8的预柱中。使用该稀释剂洗脱极性血浆成分。切换阀后，将浓缩的药物以反吹模式洗脱，并通过带有乙腈-乙酸盐缓冲液的Inertsil ODS-2色谱柱进行分离。该方法具有优异的精密度，准确性和速度，检测限为20 ng mL（-1）。每个样品的总分析时间少于40分钟，批内和批间分析的变异系数为4.8％。氯沙坦口服给药于大鼠血浆样品后，该方法已成功应用。 [参考：19]

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