首页> 外文期刊>The journal of physical chemistry, A. Molecules, spectroscopy, kinetics, environment, & general theory >Structural Transitions of Electrosprayed Ubiquitin Ions Stored in an Ion Trap over ~10 ms to 30 s
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Structural Transitions of Electrosprayed Ubiquitin Ions Stored in an Ion Trap over ~10 ms to 30 s

机译:离子阱中电喷雾泛素离子在约10 ms至30 s内的结构转变

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Structural transitions of the +6 to +8 charge states of ubiquitin produced by electrospray ionization have been studied in the gas phase by new ion tra/ion mobility-mass spectrometry technique. The approach allows transitions to be examined in detail over ~10 ms to 30 s trapping times. This time regime is intermediate between the ~1 to 5 ms time scales of previous mobility measurements [J. Am. Soc. Mass Spectrum. 1997, 8, 954] and minute to hour time scale measurements associated with trapping experiments done in a Fourier transform mass spectrometer [Int. J. Mass Spectrum. 1999, 185/186/187, 565]. The results show that over the entire time range, the +6 charge state is dominated by compact structures (with cross sections that are near the value expected for folded states in solution). The +7 state shows evidence for at least two types of initial compact structures. One state (~65% of the population) rapidly unfolds to partially folded and elongated conformers after ~30 to 40 ms. The remaining 35% of ions also unfolds at a much slower rate. The +8 charge state appears to be formed initially in a range of partially folded states. These states rapidly unfold into elongated structures that persist to the longest trapping times that are employed. These results are compared with the longer time scale measurements, and attempts are made to correlate the features observed in the different experiments.
机译:在气相中,已经通过新的离子迁移/离子迁移率质谱技术研究了由电喷雾电离产生的泛素的+6到+8电荷态的结构转变。该方法允许在大约10 ms到30 s的捕获时间内详细检查转换。这个时间范围介于先前的迁移率测量值的约1到5 ms时间尺度之间[J.上午。 Soc。质谱。 1997,8,954]和分钟到小时的时间刻度测量与在傅立叶变换质谱仪中进行的捕集实验有关[Int。 J.质谱。 1999,185/186/187,565]。结果表明,在整个时间范围内,+ 6电荷状态主要由紧凑的结构控制(横截面接近溶液中折叠状态的预期值)。 +7状态显示至少两种类型的初始紧凑结构的证据。一种状态(约占人口的65%)在约30到40毫秒后迅速展开为部分折叠和拉长的构象子。其余35%的离子也以慢得多的速度展开。最初在部分折叠状态的范围内形成了+8电荷状态。这些状态迅速展开为细长结构,并持续使用了最长的捕获时间。将这些结果与更长的时间刻度测量结果进行了比较,并尝试关联了在不同实验中观察到的特征。

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