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Study of streptavidin-modified quantum dots by capillary electrophoresis

机译：链霉亲和素修饰的量子点的毛细管电泳研究

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Great boom of nanotechnologies impacts almost all areas of science and therefore detail understanding of the properties of nanomaterials as well as their interaction abilities is required. Surface modification and functionalization of nanoparticles is of a great interest due to the wide range of applications in the area of nanomedicine, nanobiology, and/or biochemistry. In this study, CdTe QDs were synthesized using microwave reactor and their surface was modified by streptavidin to ensure further suitability for bioconjugation with biotin-labelled oligonucleotides. For characterization of the synthesized QDs and for monitoring of the interaction with the oligonucleotide, capillary and gel electrophoresis was used. Moreover, complementary advantages of absorption (CE-UV) and laser-induced fluorescence detection (CE-LIF) were exploited. Comparison the electrophoretic mobilities obtained for streptavidin-modified QDs by CE-LIF (-9.87 × 10~(-9) m~2/V/s) and by CE-UV (-10.02 × 10~(-9) m ~2/V/s) was in a good agreement enabling us to identify the peak of streptavidin-modified QDs in the CE-UV electropherogram containing also the peak of unreacted streptavidin. Subsequent conjugation of streptavidin-modified QDs with two model biotinylated oligonucleotides (BCL-2 and HBV) led to formation of the complex represented in the electropherograms as a very sharp peak. This peak height increased with time for 15.5 and 27 mAU using BCL-2 oligonucleotide and HBV oligonucleotide, respectively during 30 min interaction.
机译：纳米技术的蓬勃发展几乎影响了所有科学领域，因此需要详细了解纳米材料的性质及其相互作用能力。由于纳米药物，纳米生物学和/或生物化学领域的广泛应用，纳米粒子的表面改性和功能化引起了人们的极大兴趣。在这项研究中，使用微波反应器合成了CdTe QD，并通过链霉亲和素对其表面进行了修饰，以确保进一步适合与生物素标记的寡核苷酸进行生物缀合。为了表征合成的QD并监测与寡核苷酸的相互作用，使用了毛细管电泳和凝胶电泳。此外，还利用了吸收（CE-UV）和激光诱导荧光检测（CE-LIF）的互补优势。比较CE-LIF（-9.87×10〜（-9）m〜2 / V / s）和CE-UV（-10.02×10〜（-9）m〜2对链霉亲和素修饰的量子点的电泳迁移率/ V / s）的一致性很好，使我们能够在CE-UV电泳图中确定链霉亲和素修饰的QD的峰，其中还包含未反应的链霉亲和素的峰。随后链霉亲和素修饰的QD与两个模型生物素化寡核苷酸（BCL-2和HBV）结合，导致形成了电泳图中非常尖锐的峰。在30分钟的相互作用中，分别使用BCL-2寡核苷酸和HBV寡核苷酸，该峰高随时间分别增加15.5和27 mAU。

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