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首页> 外文期刊>Biomolecular engineering >Effect of low oxygen concentrations on growth and alpha-amylase production of Aspergillus oryzae in model solid-state fermentation systems
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Effect of low oxygen concentrations on growth and alpha-amylase production of Aspergillus oryzae in model solid-state fermentation systems

机译：低氧浓度对模型固态发酵系统中米曲霉生长和α-淀粉酶产生的影响

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摘要

Oxygen transfer in the fungal mat is a major concern in solid-state fermentation (SSF). Oxygen supply into the mycelial layers is hampered by diffusion limitation. For aerobic fungi, like Aspergillus oryzae, this oxygen depletion can be a severely limiting factor for growth and metabolite production. This paper describes the effects of a low oxygen concentration on growth at the levels of individual hyphae, colonies and overcultures, and on alpha-amylase production in overcultures. PDA medium was used to study the effect of a low oxygen concentration on hyphal elongation rate and branching frequency of hyphae, and radial extension rate of colonies of A. oryzae. We found similar saturation constants (K-O2) of 0-1% (v/v in the gas phase) for oxygen concentration described with Monod kinetics, for branching frequency of hyphae and colony extension rate. When A. oryzae was grown as an over-culture on wheat-flour model substrate at 0.25% (v/v) oxygen concentration, the reduction in growth was more pronounced than as individual hyphae and a colony on PDA medium. Experimental results also showed that the specific a-amylase production rate under the condition of 0.25% (v/v) oxygen was reduced. Because the value of K-O2 is relatively low, it is reasonable to simplify the kinetics of growth of A. oryzae to zero-order kinetics in coupled diffusion/reaction models. (c) 2005 Elsevier B.V. All rights reserved.
机译：真菌垫中的氧气转移是固态发酵（SSF）中的主要问题。扩散限制阻碍了向菌丝体层中的氧气供应。对于有氧真菌，如米曲霉，这种耗氧可能是生长和代谢产物的严重限制因素。本文介绍了低氧浓度对单个菌丝，菌落和过度培养水平下的生长以及过度培养中α-淀粉酶产生的影响。用PDA培养基研究低氧浓度对米曲霉菌丝的菌丝伸长率和分支频率以及菌落径向扩展率的影响。对于菌丝的分支频率和菌落扩展速率，我们发现用Monod动力学描述的氧浓度为0-1％（气相中的v / v）相似的饱和常数（K-O2）。当米曲霉在0.25％（v / v）的氧气浓度下作为过度培养物生长在小麦粉模型基质上时，生长的减少比PDA培养基上的单个菌丝和菌落更为明显。实验结果还表明，在0.25％（v / v）氧气的条件下，特定α-淀粉酶的生产率降低了。由于K-O2的值相对较低，因此在耦合扩散/反应模型中将米曲霉的生长动力学简化为零级动力学是合理的。 （c）2005 Elsevier B.V.保留所有权利。

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