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首页> 外文期刊>The Journal of Nutritional Biochemistry >Circulating isoflavonoid levels in CD-1 mice: effect of oral versus subcutaneous delivery and frequency of administration.
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Circulating isoflavonoid levels in CD-1 mice: effect of oral versus subcutaneous delivery and frequency of administration.

机译：CD-1小鼠中异黄酮的循环水平：口服与皮下给药的关系以及给药频率。

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The CD-1 mouse is a commonly used animal model to understand the biological effects of early-life exposure to soy isoflavones in infants. Most studies using CD-1 mice have administered isoflavones by daily subcutaneous injection, while infants receive oral feeds every few hours. The study objectives were to compare the total serum levels of genistein (GEN), daidzein (DAI) and the DAI metabolites equol and O-desmethyl-angolensin (O-DMA), after subcutaneous injection and oral dosing and to determine if frequency of oral administration results in different circulating levels of isoflavones using the CD-1 mouse model. From postnatal days 1 to 5, pups randomly received corn oil or soy isoflavones (total daily dose, 0.010 mg DAI+0.025 mg GEN) by subcutaneous injection once a day, orally once a day or orally every 4 hours. On postnatal day 5, 1 h posttreatment, mice were killed and serum was collected. Mice treated with soy isoflavones had higher (P<.05) serum GEN (female: 1895-3391 ng/ml and male: 483-578 ng/ml) and DAI (female: 850-1580 ng/ml and male: 248-322 ng/ml) concentrations versus control (5-20 ng/ml) mice, regardless of route or frequency of administration, and were similar among dosing strategies. Total serum concentrations of GEN and DAI were higher (P<.05) among females (GEN: 2714 +or- 393 ng/ml and DAI: 1205 +or- 164 ng/ml) than males (GEN: 521 +or- 439 ng/ml and DAI: 288 +or- 184 ng/ml) across treatment groups. Serum equol and O-DMA concentrations were negligible (<3 ng/ml) across groups. In conclusion, different routes of delivery and frequency of administration resulted in similar total serum levels of GEN, DAI, equol or O-DMA
机译：CD-1小鼠是一种常用的动物模型，用于了解婴儿早期接触大豆异黄酮的生物学效应。大多数使用CD-1小鼠的研究都通过每天皮下注射来给药异黄酮，而婴儿每隔几个小时就接受口服饲料。研究目的是在皮下注射和口服给药后比较染料木黄酮（GEN），大豆苷元（DAI）和DAI代谢物雌马酚和O-去甲基血管紧张素（O-DMA）的总血清水平，并确定是否口服使用CD-1小鼠模型，给药后异黄酮循环水平不同。从出生后的第1天到第5天，幼犬每天皮下注射一次，每天一次口服，或每4小时一次口服，随机接受玉米油或大豆异黄酮（每日总剂量，0.010 mg DAI + 0.025 mg GEN）。在出生后第5天，治疗后1小时，处死小鼠并收集血清。大豆异黄酮处理的小鼠的GEN（女性：1895-3391 ng / ml，男性：483-578 ng / ml）和DAI（女性：850-1580 ng / ml，男性：248-）较高（P <.05） 322 ng / ml）的浓度相对于对照（5-20​​ ng / ml）的小鼠，无论给药途径或给药频率如何，在给药策略中相似。女性（GEN：2714 +或393 ng / ml和DAI：1205 +或164 ng / ml）的GEN和DAI总血清浓度高于男性（GEN：521 +或439）（P <.05） ng / ml和DAI：288 +或184 ng / ml）。各组的血清雌马酚和O-DMA浓度可忽略不计（<3 ng / ml）。总之，不同的给药途径和给药频率导致总血清总水平的GEN，DAI，雌马酚或O-DMA相似

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