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首页> 外文期刊>The Journal of Nutritional Biochemistry >Mechanisms involved in down-regulation of intestinal IgA in rats by high cocoa intake.
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Mechanisms involved in down-regulation of intestinal IgA in rats by high cocoa intake.

机译：高可可摄入引起大鼠肠IgA下调的机制。

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Previous studies have shown that rat intestinal immunoglobulin A (IgA) concentration and lymphocyte composition of the intestinal immune system were influenced by a highly enriched cocoa diet. The aim of this study was to dissect the mechanisms by which a long-term high cocoa intake was capable of modifying gut secretory IgA in Wistar rats. After 7 weeks of nutritional intervention, Peyer's patches, mesenteric lymph nodes and the small intestine were excised for gene expression assessment of IgA, transforming growth factor beta, C-C chemokine receptor-9 (CCR9), interleukin (IL)-6, CD40, retinoic acid receptors (RAR alpha and RAR beta), C-C chemokine ligand (CCL)-25 and CCL28 chemokines, polymeric immunoglobulin receptor and toll-like receptors (TLR) expression by real-time polymerase chain reaction. As in previous studies, secretory IgA concentration decreased in intestinal wash and fecal samples after cocoa intake. Results from the gene expression showed that cocoa intake reduced IgA and IL-6 in Peyer's patches and mesenteric lymph nodes, whereas in small intestine, cocoa decreased IgA, CCR9, CCL28, RAR alpha and RAR beta. Moreover, cocoa-fed animals presented an altered TLR expression pattern in the three compartments studied. In conclusion, a high-cocoa diet down-regulated cytokines such as IL-6, which is required for the activation of B cells to become IgA-secreting cells, chemokines and chemokine receptors, such as CCL28 and CCR9 together with RAR alpha and RAR beta, which are involved in the gut homing of IgA-secreting cells. Moreover, cocoa modified the cross-talk between microbiota and intestinal cells as was detected by an altered TLR pattern. These overall effects in the intestine may explain the intestinal IgA down-regulatory effect after the consumption of a long-term cocoa-enriched diet
机译：先前的研究表明，大鼠肠道免疫球蛋白A（IgA）的浓度和肠道免疫系统的淋巴细胞组成受高浓可可饮食的影响。这项研究的目的是剖析长期高可可摄入量能够改变Wistar大鼠肠道分泌IgA的机制。营养干预7周后，切除Peyer斑块，肠系膜淋巴结和小肠，以评估IgA，转化生长因子β，CC趋化因子受体9（CCR9），白介素（IL）-6，CD40，视黄酸的基因表达通过实时聚合酶链式反应表达酸性受体（RAR alpha和RAR beta），CC趋化因子配体（CCL）-25和CCL28趋化因子，聚合免疫球蛋白受体和toll样受体（TLR）。与以前的研究一样，可可摄入后肠洗液和粪便中的分泌型IgA浓度降低。基因表达的结果表明，可可摄入减少了Peyer斑块和肠系膜淋巴结的IgA和IL-6，而在小肠中，可可减少了IgA，CCR9，CCL28，RARα和RARβ。此外，可可喂养的动物在所研究的三个部分中表现出改变的TLR表达模式。总之，高可可饮食下调了细胞因子，例如IL-6，这是激活B细胞成为分泌IgA的细胞，趋化因子和趋化因子受体（例如CCL28和CCR9）以及RAR alpha和RAR所必需的β，与分泌IgA的肠道归巢有关。此外，可可改变了微生物群和肠道细胞之间的串扰，这是通过改变的TLR模式检测到的。这些对肠道的总体影响可能解释了长期食用富含可可粉的饮食对肠道IgA的下调作用

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