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首页> 外文期刊>The Journal of Nutritional Biochemistry >Murine CD8+ T cells but not macrophages express the vitamin D 1 alpha -hydroxylase.
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Murine CD8+ T cells but not macrophages express the vitamin D 1 alpha -hydroxylase.

机译：鼠CD8 + T细胞但不表达巨噬细胞表达维生素D 1α-羟化酶。

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摘要

The active form of vitamin D, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] is synthesized by the 1 alpha -hydroxylase, which is encoded by the Cyp27B1 gene. Using transgenic mice that have replaced the Cyp27B1 gene with the bacterial lacZ reporter gene ( beta -galactosidase), the inflammatory conditions that induce Cyp27B1 in the immune system were probed. A variety of stimuli including lipopolysaccharide, anti-CD3 or PMA/ionomycin were used to stimulate splenocytes and bone marrow derived macrophage in vitro. Only anti-CD3 stimulation resulted in a low induction of beta -galactosidase activity in the spleen, indicating that T cells might be a source of Cyp27B1. In vivo, challenge with lipopolysaccharide, alpha -galactosylceramide, and Listeria monocytogenes failed to induce beta -galactosidase activity outside of the kidneys. During more prolonged and severe inflammation there was staining in both the lungs and the gastrointestinal tract for beta -galactosidase. Furthermore, wild-type reconstitution of the hematopoietic cell population in Cyp27B1 KO mice protected the mice from experimental colitis. T cell production of Cyp27B1 activity was shown to be from the CD8+ but not the CD4+T cell population. CD8+T cells expressed the reporter gene only after 48 h of stimulation. The data is consistent with a model where CD8+T cells are activated to produce Cyp27B1 and 1,25(OH)2D3 that serves to turn off the local immune response.
机译：维生素D的活性形式是1,25-二羟基维生素D 3 [1,25（OH） 2 D 3 ]的合成。 1个α-羟化酶，由Cyp27B1基因编码。使用已用细菌lacZ报告基因（β-半乳糖苷酶）替代Cyp27B1基因的转基因小鼠，检测了在免疫系统中诱导Cyp27B1的炎症条件。包括脂多糖，抗CD3或PMA /离子霉素在内的多种刺激物可在体外刺激脾细胞和骨髓衍生的巨噬细胞。只有抗CD3刺激导致脾脏中β-半乳糖苷酶活性的诱导降低，表明T细胞可能是Cyp27B1的来源。在体内，用脂多糖，α-半乳糖基神经酰胺和单核细胞增生李斯特氏菌刺激，无法在肾脏外部诱导β-半乳糖苷酶活性。在更长时间和更严重的炎症过程中，肺和胃肠道均有β-半乳糖苷酶染色。此外，Cyp27B1 KO小鼠中造血细胞群体的野生型重建可以保护小鼠免受实验性结肠炎的侵害。显示Cyp27B1活性的T细胞产生来自CD8 +，而不是CD4 + T细胞群体。 CD8 + T细胞仅在刺激48小时后才表达报告基因。该数据与激活CD8 + T细胞以产生Cyp27B1和1,25（OH） 2 D 3 来关闭局部免疫反应的模型一致。

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