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首页> 外文期刊>The Journal of Nutritional Biochemistry >Comparative metabolomics approach coupled with cell- and gene-based assays for species classification and anti-inflammatory bioactivity validation of Echinacea plants.
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Comparative metabolomics approach coupled with cell- and gene-based assays for species classification and anti-inflammatory bioactivity validation of Echinacea plants.

机译：比较代谢组学方法与基于细胞和基因的测定方法相结合，用于紫锥菊科植物的物种分类和抗炎生物活性验证。

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摘要

Echinacea preparations were the top-selling herbal supplements or medicines in the past decade; however, there is still frequent misidentification or substitution of the Echinacea plant species in the commercial Echinacea products with not well chemically defined compositions in a specific preparation. In this report, a comparative metabolomics study, integrating supercritical fluid extraction, gas chromatography/mass spectrometry and data mining, demonstrates that the three most used medicinal Echinacea species, Echinacea purpurea, E. pallida, and E. angustifolia, can be easily classified by the distribution and relative content of metabolites. A mitogen-induced murine skin inflammation study suggested that alkamides were the active anti-inflammatory components present in Echinacea plants. Mixed alkamides and the major component, dodeca-2E,4E,8Z,10Z(E)-tetraenoic acid isobutylamides (8/9), were then isolated from E. purpurea root extracts for further bioactivity elucidation. In macrophages, the alkamides significantly inhibited cyclooxygenase 2 (COX-2) activity and the lipopolysaccharide-induced expression of COX-2, inducible nitric oxide synthase and specific cytokines or chemokines [i.e., TNF- alpha , interleukin (IL)-1 alpha , IL-6, MCP-1, MIP-1 beta ] but elevated heme oxygenase-1 protein expression. Cichoric acid, however, exhibited little or no effect. The results of high-performance liquid chromatography/electron spray ionization/mass spectrometry metabolite profiling of alkamides and phenolic compounds in E. purpurea roots showed that specific phytocompound (i.e., alkamides, cichoric acid and rutin) contents were subject to change under certain post-harvest or abiotic treatment. This study provides new insight in using the emerging metabolomics approach coupled with bioactivity assays for medicinalutritional plant species classification, quality control and the identification of novel botanical agents for inflammatory disorders.
机译：紫锥菊制剂是过去十年中最畅销的草药补品或药物;然而，在特定制剂中化学成分不明确的商业化松果菊属产品中，松果菊属植物物种仍然经常被误判或替代。在这份报告中，一项比较代谢组学研究将超临界流体提取，气相色谱/质谱和数据挖掘相结合，证明了三种最常用的药用紫锥菊属物种紫锥菊， E。帕利达（Pallida）和 E。可以通过代谢物的分布和相对含量轻松地将其分类。丝裂原诱导的鼠皮肤炎症研究表明，链酰胺是紫锥菊中的活性抗炎成分。混合链烷酰胺及其主要成分dodeca-2 E ，4 E ，8 Z ，10 Z （<然后从 E中分离出i> E ）-四烯酸异丁酰胺（8/9）。紫根提取物用于进一步阐明生物活性。在巨噬细胞中，烷酰胺显着抑制环氧合酶2（COX-2）的活性以及脂多糖诱导的COX-2，诱导型一氧化氮合酶和特定细胞因子或趋化因子[即TNF-α，白介素（IL）-1α， IL-6，MCP-1，MIP-1β]，但血红素加氧酶-1蛋白表达升高。然而，次氯酸几乎没有作用。高效液相色谱/电子喷雾电离/质谱法分析E中链烷酰胺和酚类化合物的代谢产物的结果。紫菜根表明，在某些收获后或非生物处理下，特定的植物化合物（即链烷酰胺，次氯酸和芦丁）的含量会发生变化。这项研究为使用新兴的代谢组学方法以及生物活性测定方法进行药用/营养性植物物种分类，质量控制和鉴定炎症性新型植物药提供了新的见识。

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