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首页> 外文期刊>The Journal of Nutritional Biochemistry >Plant sterol and stanol substrate specificity of pancreatic cholesterol esterase
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Plant sterol and stanol substrate specificity of pancreatic cholesterol esterase

机译：胰腺胆固醇酯酶的植物甾醇和甾烷醇底物特异性

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摘要

Consumption of plant sterols or stanols (collectively referred to as phytosterols) and their esters results in decreased low-density lipoprotein cholesterol, which is associated with decreased atherosclerotic risk. The mechanisms by which phytosterols impart their effects, however, are incompletely characterized. The objective of the present study is to determine if pancreatic cholesterol esterase (PCE; EC 3.1.1.13), the enzyme primarily responsible for cholesterol ester hydrolysis in the digestive tract, is capable of hydrolyzing various phytosterol esters and to compare the rates of sterol ester hydrolysis in vitro. We found that PCE hydrolyzes palmitate, oleate and stearate esters of cholesterol, stigmasterol, stigmastanol and sitosterol. Furthermore, we found that the rate of hydrolysis was dependent on both the sterol and the fatty acid moieties in the following order of rates of hydrolysis: cholesterol>(sitosterol=stigmastanol)>stigmasterol; oleate>(palmitate=stearate). The addition of free phytosterols to the system did not change hydrolytic activity of PCE, while addition of palmitate, oleate or stearate increased activity. Thus, PCE may play an important but discriminatory role in vivo in the liberation of free phytosterols to compete with cholesterol for micellar solubilization and absorption
机译：食用植物固醇或甾烷醇（统称为植物固醇）及其酯类会导致低密度脂蛋白胆固醇降低，从而降低动脉粥样硬化风险。然而，植物甾醇发挥作用的机理尚未完全阐明。本研究的目的是确定主要负责消化道中胆固醇酯水解的酶胰胆固醇酯酶（PCE; EC 3.1.1.13）是否能够水解各种植物甾醇酯并比较甾醇酯的比率体外水解。我们发现PCE可水解胆固醇，豆甾醇，stigmastanol和谷甾醇的棕榈酸酯，油酸酯和硬脂酸酯。此外，我们发现水解速率在以下水解速率顺序上取决于甾醇和脂肪酸部分：胆固醇>（谷固醇= stigmastanol）>豆甾醇;油酸酯>（棕榈酸酯=硬脂酸酯）。向系统中添加游离植物甾醇不会改变PCE的水解活性，而添加棕榈酸酯，油酸酯或硬脂酸酯则提高了活性。因此，PCE在体内释放游离植物甾醇以与胆固醇竞争胶束增溶和吸收方面可能起重要但有歧视作用

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