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首页> 外文期刊>Biomolecular engineering >Early stages of human plasma proteins adsorption probed by Atomic Force Microscope.
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Early stages of human plasma proteins adsorption probed by Atomic Force Microscope.

机译：原子力显微镜探测人血浆蛋白吸附的早期阶段。

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摘要

Atomic Force Microscope (AFM) as a surface characterization technique has offered a great impulse in the advance of biocompatible materials. In this study AFM was implemented for the investigation of the early stages of adsorption of two human plasma proteins on titanium and hydrogenated carbon biocompatible thin films. The plasma proteins that were used were Human Serum Albumin and Fibrinogen, two of the most important proteins in human plasma. The concentration of the protein solutions was the same as that in human plasma. As the examined samples were soft, non-contact AFM mode was used to avoid their destruction. In order for the early stages of protein adsorption to be assessed, small incubation times were applied. AFM measurements in liquid buffer were also carried out, allowing the observation of the protein behaviour in an environment much closer to their native one. In addition, there was an assessment of the adsorption mechanism of the proteins on the above-mentioned biomaterials.
机译：原子力显微镜（AFM）作为一种表面表征技术，为生物相容性材料的发展提供了极大的推动力。在这项研究中，AFM用于研究两种人类血浆蛋白在钛和氢化碳生物相容性薄膜上的早期吸附过程。使用的血浆蛋白是人血清白蛋白和纤维蛋白原，这是人血浆中最重要的两种蛋白质。蛋白质溶液的浓度与人血浆中的浓度相同。由于所检查的样品较软，因此使用非接触式AFM模式以避免其破坏。为了评估蛋白质吸附的早期阶段，应使用较小的孵育时间。还可以在液体缓冲液中进行AFM测量，从而可以在更接近其天然环境的环境中观察蛋白质的行为。另外，还评估了蛋白质在上述生物材料上的吸附机理。

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