• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomolecular engineering >Emulsion-based synthesis of PLGA-microspheres for the in vitro expansion of porcine chondrocytes
【24h】

Emulsion-based synthesis of PLGA-microspheres for the in vitro expansion of porcine chondrocytes

机译：基于乳液的PLGA微球的合成，用于猪软骨细胞的体外扩增

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The in vitro cell expansion of autologouschondrocytes is of high interest in regenerative medicine since these cell scan be used to treat joint cartilage defects. In order to preserve chondrocyte phenotype, while optimizing adhesion on microspheres, several processing parameters for the microsphere synthesis were varied. In this study three different polylactide-co-glycolides were used with differing lactide-glycolide ratios (85:15 and 50:50) and differing inherent viscosities. An emulsion route was established, where the polymer was dissolved in chloroform and then injected into a stirred polyvinyl alcohol-water solution at different polymer concentrations and different stirring velocities to produce microspheres with varying diameters. The sphere size distribution and morphology was analyzed using image processing software on SEM pictures. Based on previous experiments with commercial microspheres, three optimum samples were selected for further investigations. The degradation of the microspheres was determined in a long-term experiment in culture medium for 3 months. Adherent cells were characterized after 3 and 5 days by FDA + EB vital staining and in SEM. (C) 2007 Elsevier B.V. All rights reserved.
机译：自体软骨细胞的体外细胞扩增在再生医学中备受关注，因为这些细胞扫描可用于治疗关节软骨缺损。为了保留软骨细胞表型，同时优化在微球上的附着力，改变了微球合成的几个加工参数。在这项研究中，使用了三种不同的丙交酯-共-乙交酯，丙交酯-乙交酯的比例不同（85:15和50:50），固有粘度也不同。建立了乳液路线，其中将聚合物溶解在氯仿中，然后以不同的聚合物浓度和不同的搅拌速度注入搅拌的聚乙烯醇-水溶液中，以生产直径不同的微球。使用图像处理软件在SEM图片上分析了球体的大小分布和形态。根据先前使用商业微球的实验，选择了三个最佳样品进行进一步研究。微球的降解是在培养基中长期实验3个月后确定的。在第3天和第5天后，通过FDA + EB活力染色和SEM对贴壁细胞进行表征。 （C）2007 Elsevier B.V.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号