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Evaluating the effect of soft lining materials on the growth of yeast.

机译:评估软衬材料对酵母生长的影响。

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STATEMENT OF PROBLEM: Soft lining materials continue to have a place in clinical removable prosthodontics. However, there is an increased probability of yeast colonization on soft lining materials. PURPOSE: This study (1) assessed a method of evaluating the effect of long-term soft lining materials on the growth of yeast and (2) investigated the effect five soft lining materials had on the growth of three species of yeast. MATERIAL AND METHODS: Coe Supersoft, Novus, and three experimental soft lining materials were investigated together with Candida albicans, Candida tropicalis, and Issatchenkia orientalis (formerly Candida krusei) yeasts. Strips of soft lining material incubated on blood agar plates were examined for inhibition of the growth of yeast. Soft lining materials soaked in sterile trypticase soya broth or water were inoculated with yeast and incubated. The change in colony forming units per milliliter from the initial load of yeast at 3 days was measured. Statistical analysis was performed with an independent paired Student t test. RESULTS: Inhibition of yeast growth occurred for two soft lining materials. Despite the presence of sufficient viable organisms, differences between the initial load of yeast and the 3-day results were mostly small, both for the test and control groups, suggesting that the material does not support the growth of the tested yeast during this period. CONCLUSIONS: The often described increased prevalence of yeast associated with soft lining materials in the oral environment is likely related to readily available nutrients in the mouth and the difficulty in maintaining and cleaning these materials.
机译:问题陈述:软衬材料在临床可移动义齿修复中继续占有一席之地。但是,酵母菌在软衬材料上定殖的可能性增加。目的:本研究(1)评估了评估长期软衬材料对酵母生长的影响的方法,(2)研究了5种软衬材料对三种酵母的生长的影响。材料与方法:研究了Coe Supersoft,Novus和三种实验性软衬里材料,以及白色念珠菌,热带念珠菌和东方伊莎氏酵母(以前称为假丝酵母)酵母。检查在血琼脂板上温育的软衬里材料条对酵母菌生长的抑制作用。将浸泡在无菌胰蛋白tic大豆肉汤或水中的软衬里材料接种酵母并孵育。测量了从第3天酵母的初始负荷起每毫升菌落形成单位的变化。使用独立的成对学生t检验进行统计分析。结果:两种软衬材料均抑制了酵母的生长。尽管存在足够的活生物体,但对于测试组和对照组,酵母的初始负荷和3天结果之间的差异大多很小,这表明该材料在此期间不支持测试酵母的生长。结论:经常描述的与口腔环境中柔软衬里材料有关的酵母菌流行率增加可能与口腔中容易获得的营养物质以及维护和清洁这些材料的难度有关。

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