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首页> 外文期刊>The Korean Journal of Parasitology >Prevalence of Haplorchis taichui in field-collected snails: a molecular approach.
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Prevalence of Haplorchis taichui in field-collected snails: a molecular approach.

机译：田间田螺中 Haplorchis taichui 的流行：一种分子方法。

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摘要

The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda: Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata, Tarebia granifera, and Thiara scabra. The overall prevalence of pleurolophocercous cercariae was 21.7% as determined by the morphology. For genetic detection of H. taichui infection in snails, 2 primers Hapt_F (5'-GGCCAACGCAATCGTCATCC-3') and Hapt_R (5'-GCGTCGGGTTTCAGACATGG-3'), were used. The genomic DNA of H. taichui, which was used as a positive control, gave an amplification of the 256 bp fragment. The overall prevalence of H. taichui from specific PCR was 9.7%. The proportion of H. taichui among the pleurolophocercous cercariae in this study was 44.9%.
机译：使用光学显微镜和物种特异性PCR方法，研究了噻虫（Gastropoda：Thiaridae）中肠道吸虫 Haplorchis taichui 的子宫颈阶段的患病率。从泰国北部清迈府湄登地区共采集了988只蜗牛，其中包括3种。 Melanoides tuberculata ， Tarebia granifera 和 Thiara scabra 。通过形态学确定，尾pho癌的总患病率为21.7％。用于H的基因检测。蜗牛的taichui感染，使用了两个引物Hapt_F（5'-GGCCAACGCAATCGTCATCC-3'）和Hapt_R（5'-GCGTCGGGTTTCAGACATGG-3'）。 H的基因组DNA。用作阳性对照的taichui 扩增了256bp的片段。 H的总体患病率。特异性PCR得到的tichui 为9.7％。 H的比例。在这项研究中，尾pho中的taichui为44.9％。

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