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首页> 外文期刊>The New Phytologist >A metabolic gene cluster in Lotus japonicus discloses novel enzyme functions and products in triterpene biosynthesis
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A metabolic gene cluster in Lotus japonicus discloses novel enzyme functions and products in triterpene biosynthesis

机译：莲花中的代谢基因簇揭示了三萜生物合成中的新酶功能和产物

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摘要

Genes for triterpene biosynthetic pathways exist as metabolic gene clusters in oat and Arabidopsis thaliana plants. We characterized the presence of an analogous gene cluster in the model legume Lotus japonicus. In the genomic regions flanking the oxidosqualene cyclase AMY2 gene, genes for two different classes of cytochrome P450 and a gene predicted to encode a reductase were identified. Functional characterization of the cluster genes was pursued by heterologous expression in Nicotiana benthamiana.The gene expression pattern was studied under different developmental and environmental conditions. The physiological role of the gene cluster in nodulation and plant development was studied in knockdown experiments. A novel triterpene structure, dihydrolupeol, was produced by AMY2. A new plant cytochrome P450, CYP71D353, which catalyses the formation of 20-hydroxybetulinic acid in a sequential three-step oxidation of 20-hydroxylupeol was characterized. The genes within the cluster are highly co-expressed during root and nodule development, in hormone-treated plants and under various environmental stresses. A transcriptional gene silencing mechanism that appears to be involved in the regulation of the cluster genes was also revealed. A tightly co-regulated cluster of functionally related genes is involved in legume triterpene biosynthesis, with a possible role in plant development.
机译：三萜生物合成途径的基因以代谢基因簇的形式存在于燕麦和拟南芥植物中。我们表征了豆科植物日本莲花模型中类似基因簇的存在。在氧化角鲨烯环化酶AMY2基因侧翼的基因组区域中，鉴定了两种不同类型的细胞色素P450的基因和预计编码还原酶的基因。本研究通过对本生烟草的异源表达进行聚类基因的功能表征。研究了不同发育和环境条件下基因表达的规律。在敲除实验中研究了基因簇在结瘤和植物发育中的生理作用。 AMY2生产了一种新颖的三萜结构，二氢卢波醇。表征了一种新的植物细胞色素P450 CYP71D353，它在20-羟基卢比醇的连续三步氧化过程中催化20-羟基贝丁酸的形成。在激素处理过的植物中以及在各种环境胁迫下，簇中的基因在根和根瘤发育过程中高度共表达。还揭示了似乎与簇基因调控有关的转录基因沉默机制。功能相关基因的紧密调控簇与豆类三萜生物合成有关，可能在植物发育中起作用。

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