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首页> 外文期刊>The Progressive Fish-Culturist >USE OF CELLULOSE ACETATE ELECTROPHORESIS TO RAPIDLY SCREEN SAUGER BROODSTOCK FOR SAUGER-WALLEYE HYBRIDS
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USE OF CELLULOSE ACETATE ELECTROPHORESIS TO RAPIDLY SCREEN SAUGER BROODSTOCK FOR SAUGER-WALLEYE HYBRIDS

机译：醋酸纤维素电泳法快速筛查索格-沃利混合动力索格菌素

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摘要

To maintain the genetic integrity of their product, hatchery managers and culturists often need to rapidly screen fish that are intended as broodstock for hybridization with closely related species. During March 1995 and 1996, we evaluated the use of cellulose acetate electrophoresis to screen wild saugers Stizostedion canadense from the Peoria Pool of the Illinois River for hybridization with walleyes S. vitreum. Seven of 372 fish (1.9%) in 1995 and 30 of 733 fish (4.1%) in 1996 that had been identified as saugers, based on external morphology and skin pigmentation, were subsequently identified by electrophoresis as sauger-walleye hybrids and were excluded from the breeding program. In addition, polymorphisms at the phosphoglucomutase locus PGM-1* were identified among Illinois River saugers. Cellulose acetate electrophoresis allowed rapid (<30 min) discrimination of saugers from hybrids and promises to be a highly efficient system for screening potential broodstock in a hatchery environment.
机译：为了保持其产品的遗传完整性，孵化场的管理者和养殖者通常需要快速筛查用作亲鱼的鱼类，以便与紧密相关的物种杂交。在1995年3月和1996年期间，我们评估了使用醋酸纤维素电泳筛选伊利诺伊州皮奥里亚河池中野生木枝甜菜的能力，以便与玻璃眼葡萄球菌杂交。根据外部形态和皮肤色素沉着，在1995年的372条鱼中有7条（1.9％），在1996年的733条鱼中有30条（4.1％）随后被电泳鉴定为索格-壁眼杂种，被排除在外。育种程序。此外，在伊利诺伊州河纵木中发现了磷酸葡萄糖突变酶基因座PGM-1 *的多态性。醋酸纤维素电泳可以快速（<30分钟）区分杂种中的sa，并且有望成为在孵化场环境中筛选潜在亲鱼的高效系统。

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