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首页> 外文期刊>The Journal of Experimental Biology >CHANGES IN NA+/K+-ATPASE EXPRESSION DURING ADAPTIVE CELL DIFFERENTIATION IN AVIAN NASAL SALT GLAND
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CHANGES IN NA+/K+-ATPASE EXPRESSION DURING ADAPTIVE CELL DIFFERENTIATION IN AVIAN NASAL SALT GLAND

机译：禽鼻盐腺适应性细胞分化过程中NA + / K + -ATPase表达的变化

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Chronic salt stress in ducklings (Anas platyrhynchos) resulted in a sustained accumulation of cyclic AMP in the secretory cells of the nasal salt glands, Adaptive increases in the activity of the Na+/K+-ATPase, measured as ATP hydrolysis rates in freshly isolated tissue, were observed after 12 h of salt stress, This change in enzyme activity was associated with increases in protein abundance in the alpha- as well as in the beta-subunit of Na+/K+-ATPase and an increase in beta-subunit glycosylation, We investigated whether the increase in the cytosolic cyclic AMP concentration and the adaptive changes in Na+/K+-ATPase activity were causally related, Using an organotypic tissue culture system for salt gland slices from unstressed (naive) ducklings, we produced similar changes in Na+/K+-ATPase activity and subunit abundance by treating cultured tissue with drugs that elevate cytosolic cyclic AMP levels (forskolin, 8-CPT-cAMP) during a 15 h culture period, Protein synthesis assays using cultured tissue revealed that elevations in cytosolic cyclic AMP level mediate increases in Na+/K+-ATPase subunit abundance by slowing down the degradation of ATPase subunits. This increase in the amount of enzyme protein was associated with a significant increase in Na+/K+-ATPase activity in tissue homogenates, The time course of these changes in cyclic-AMP-treated cultured tissue resembled that observed in salt-stressed intact animals, indicating that the elevation in cyclic AMP level in salt gland tissue may constitute a portion of the signalling events ultimately leading to the adaptive increase in Na+/K+-ATPase activity in vivo. [References: 38]
机译：雏鸭（Anas platyrhynchos）中的慢性盐胁迫导致鼻腔腺体分泌细胞中环状AMP的持续积累，Na + / K + -ATPase活性的适应性增加（以新鲜分离的组织中的ATP水解速率衡量），在盐胁迫12小时后观察到，这种酶活性的变化与Na + / K + -ATPase的α-以及β-亚基中蛋白质丰度的增加以及β-亚基糖基化的增加有关，我们调查了胞质环AMP浓度的增加与Na + / K + -ATPase活性的适应性变化是否因果相关，使用有机型组织培养系统处理未受力（幼稚）小鸭的盐腺切片，我们产生了类似的Na + / K +-变化通过在15小时的培养期内用可提高细胞溶质环AMP含量的药物（佛司可林，8-CPT-cAMP）处理培养的组织来实现ATP酶活性和亚基的丰度，使用cu进行蛋白质合成测定破裂的组织显示，胞质环AMP含量的升高通过减缓ATPase亚基的降解来介导Na + / K + -ATPase亚基丰度的增加。酶蛋白量的这种增加与组织匀浆中Na + / K + -ATPase活性的显着增加有关。在经过环AMP处理的培养组织中，这些变化的时间过程类似于在盐胁迫的完整动物中观察到的过程，盐腺组织中环AMP含量的升高可能构成了一部分信号事件，最终导致体内Na + / K + -ATPase活性的适应性增加。 [参考：38]

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