• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Royal Society Proceedings B: Biological Sciences >A new perspective on cannabinoid signalling: complementary localization of fatty acid amide hydrolase and the CB1 receptor in rat brain.
【24h】

A new perspective on cannabinoid signalling: complementary localization of fatty acid amide hydrolase and the CB1 receptor in rat brain.

机译：大麻素信号传导的新观点：脂肪酸酰胺水解酶和CB1受体在大鼠脑中的互补定位。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

CB1-type cannabinoid receptors in the brain mediate effects of the drug cannabis. Anandamide and sn-2 arachidonylglycerol (2-AG) are putative endogenous ligands for CB1 receptors, but it is not known which cells in the brain produce these molecules. Recently, an enzyme which catalyses hydrolysis of anandamide and 2-AG, known as fatty acid amide hydrolase (FAAH), was identified in mammals. Here we have analysed the distribution of FAAH in rat brain and compared its cellular localization with CB1-type cannabinoid receptors using immunocytochemistry. High concentrations of FAAH activity were detected in the cerebellum, hippocampus and neocortex, regions of the rat brain which are enriched with cannabinoid receptors. Immunocytochemical analysis of these brain regions revealed a complementary pattern of FAAH and CB1 expression with CB1 immunoreactivity occurring in fibres surrounding FAAH-immunoreactive cell bodies and/or dendrites. In the cerebellum, FAAH was expressed in the cell bodies of Purkinje cells and CB1 was expressed in the axons of granule cells and basket cells, neurons which are presynaptic to Purkinje cells. The close correspondence in the distribution of FAAH and CB1 in rat brain and the complementary pattern of FAAH and CB1 expression at the cellular level provides important new evidence that FAAH may participate in cannabinoid signalling mechanisms of the brain.
机译：脑中的CB1型大麻素受体介导了药物大麻的作用。 Anandamide和sn-2花生四烯酸甘油酯（2-AG）是CB1受体的推定内源性配体，但尚不清楚大脑中的哪些细胞产生这些分子。最近，在哺乳动物中发现了一种催化anandamide和2-AG水解的酶，称为脂肪酸酰胺水解酶（FAAH）。在这里，我们已经分析了FAAH在大鼠脑中的分布，并使用免疫细胞化学比较了其与CB1型大麻素受体的细胞定位。在小脑，海马和新皮层（富含大麻素受体的大鼠大脑区域）中检测到高浓度的FAAH活性。这些大脑区域的免疫细胞化学分析显示，FAAH和CB1表达的互补模式与FAAH免疫反应性细胞体和/或树突周围的纤维中发生的CB1免疫反应性有关。在小脑中，FAAH在浦肯野细胞的细胞体中表达，CB1在颗粒细胞和篮状细胞（与浦肯野细胞突触的神经元）的轴突中表达。大鼠大脑中FAAH和CB1的分布密切相关，并且在细胞水平上FAAH和CB1表达的互补模式提供了重要的新证据，表明FAAH可能参与了大脑的大麻素信号传导机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号