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首页> 外文期刊>Tissue engineering >Hyaline Cartilage Regeneration Using Mixed Human Chondrocytes and Transforming Growth Factor-beta(1)- Producing Chondrocytes.
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Hyaline Cartilage Regeneration Using Mixed Human Chondrocytes and Transforming Growth Factor-beta(1)- Producing Chondrocytes.

机译：使用混合的人类软骨细胞和转化生长因子-β（1）生产软骨细胞的透明软骨再生。

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摘要

The purpose of this study was to investigate the efficacy of cartilage regeneration when using a mixture of transforming growth factor-beta(1) (TGF-beta(1))-producing human chondrocytes (hChon-TGF-beta(1)) and primary human chondrocytes (hChon) ("mixed cells"), compared with either hChon-TGF-beta(1) or hChon cells alone. Specifically, mixed cells or hChon cells were first injected intradermally into the backs of immune-deficient nude mice to test the feasibility of cartilage formation in vivo. Both the mixed cells and the hChon-TGF-beta(1) cells alone induced cartilage formation in nude mice, whereas hChon cells alone did not. To further test the efficacy of the cells in generating cartilage, an artificially induced partial thickness defect of the femoral condyle of a rabbit knee joint was loaded with hChon-TGF-beta(1) cells with or without mixing additional untransduced hChon cells, and hyaline cartilage regeneration was observed at 4 or 6 weeks. The efficiency of complete filling of the defect and the quality of tissue generated after implanting were evaluated on the basis of a histological grading system modified from O'Driscoll et al. (J. Bone Joint Surg. 70A, 595, 1988). Significantly, mixed cells (14.2 +/- 0.9) produced significantly better results than hChon-TGF-beta(1) (9.0 +/- 1.7) or hChon (8.0 +/- 1.8) cells alone. Histological and immunohistochemical staining of the newly repaired tissues produced after treatment with either mixed cells or hChon-TGF-beta(1) cells alone showed hyaline cartilage- like characteristics. These results suggest that the implantation of mixed cells may be a clinically efficient method of regenerating hyaline articular cartilage.
机译：这项研究的目的是调查使用产生转化生长因子-β（1）（TGF-β（1））的人类软骨细胞（hChon-TGF-β（1））和原代细胞的混合物时软骨再生的功效人软骨细胞（hChon）（“混合细胞”）与单独的hChon-TGF-beta（1）或hChon细胞相比。具体而言，首先将混合细胞或hChon细胞皮内注射到免疫缺陷裸鼠的背部，以测试体内软骨形成的可行性。混合细胞和hChon-TGF-beta（1）细胞都单独诱导了裸鼠的软骨形成，而单独的hChon细胞则没有。为了进一步测试细胞产生软骨的功效，将人工诱导的兔膝关节股骨partial的部分厚度缺损加载了hChon-TGF-beta（1）细胞，并混合或不混合其他未转导的hChon细胞和透明质酸在4或6周时观察到软骨再生。根据O'Driscoll等人改良的组织学分级系统，评估了缺损的完全填充效率和植入后产生的组织的质量。 （J.Bone Joint Surg.70A，595，1988）。重要的是，混合细胞（14.2 +/- 0.9）产生的结果明显优于单独的hChon-TGF-beta（1）（9.0 +/- 1.7）或hChon（8.0 +/- 1.8）细胞。组织学和免疫组化染色处理后的混合细胞或单独的hChon-TGF-beta（1）细胞单独产生的新修复的组织显示透明软骨样特征。这些结果表明，混合细胞的植入可能是再生透明关节软骨的临床有效方法。

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