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首页> 外文期刊>Toxicology in vitro: an international journal published in association with BIBRA >Lecithin in mixed micelles attenuates the cytotoxicity of bile salts in Caco-2 cells
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Lecithin in mixed micelles attenuates the cytotoxicity of bile salts in Caco-2 cells

机译：混合胶束中的卵磷脂减弱了Caco-2细胞中胆盐的细胞毒性

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This study was designed to investigate the cytotoxicity of bile salt-lecithin mixed micelles on the Caco-2 cell model. Cell viability and proliferation after mixed micelles treatments were evaluated with the MTT assay, and the integrity of Caco-2 cell monolayer was determined by quantitating the transepithelial electrical resistance and the flux of tracer, FITC-dextran 4400. The apoptosis induced by mixed micelles treatments was investigated with the annexin V/PI protocol. The particle size of mixed micelles was all smaller than 100. nm. The mixed micelles with lower than 0.2. mM sodium deoxycholate (SDC) had no significant effects on cell viability and proliferation. When the level of SDC was higher than 0.4. mM and the lecithin/SDC ratio was lower than 2:1, the mixed micelles caused significant changes in cell viability and proliferation. Furthermore, the mixed micelles affected tight junctions in a composition-dependent manner. Specifically, the tight junctions were transiently opened rather than damaged by the mixed micelles with SDC of between 0.2 and 0.6. mM. The mixed micelles with more lecithin also induced less apoptosis. These results demonstrate that relatively higher concentrations of mixed micelles are toxic to Caco-2 cells, while phospholipids can attenuate the toxicity of the bile salts. ? 2012 Elsevier Ltd.
机译：这项研究旨在研究胆盐-卵磷脂混合胶束对Caco-2细胞模型的细胞毒性。用MTT法评估混合胶束处理后的细胞活力和增殖，并通过定量上皮电阻和示踪剂FITC-葡聚糖4400的通量来确定Caco-2细胞单层的完整性。混合胶束处理诱导的细胞凋亡用Annexin V / PI协议进行了调查。混合胶束的粒径均小于100 nm。混合胶束低于0.2。 mM脱氧胆酸钠（SDC）对细胞活力和增殖没有明显影响。当SDC的水平高于0.4时。 mM和卵磷脂/ SDC比率低于2：1，混合的胶束引起细胞活力和增殖的显着变化。此外，混合的胶束以依赖成分的方式影响紧密连接。具体来说，紧密连接是暂时打开的，而不是被SDC在0.2到0.6之间的混合胶束破坏。毫米具有更多卵磷脂的混合胶束也诱导较少的细胞凋亡。这些结果表明，相对较高浓度的混合胶束对Caco-2细胞具有毒性，而磷脂可以减弱胆汁盐的毒性。 ？ 2012爱思唯尔有限公司

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