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首页> 外文期刊>Toxicology: An International Journal Concerned with the Effects of Chemicals on Living Systems >Astaxanthin inhibits cytotoxic and genotoxic effects of cyclophosphamide in mice germ cells.
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Astaxanthin inhibits cytotoxic and genotoxic effects of cyclophosphamide in mice germ cells.

机译：虾青素可抑制环磷酰胺对小鼠生殖细胞的细胞毒性和遗传毒性。

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Cyclophosphamide (CP), an alkylating agent used in the treatment of several cancers as well as an immunosuppressant in rheumatoid arthritis. It is used against several cancers due to its broad spectrum efficacy, but at the same time possesses unwanted risks for occupational exposure as well as therapy related toxicities to patients. The present study was aimed to investigate the protective effect of astaxanthin (AST) a red carotenoid pigment on CP induced germ cell toxicity in male mice. CP was administered intraperitoneally (i.p.) at the dose of 50, 100 and 200mg/kg body weight to mice (20-25 g) once in a week for a period of five weeks. AST was given at the dose of 25mg/kg per oral (p.o.) for five consecutive days in a week for five weeks. The animals were sacrificed one week after the last injection of CP. The protective effect of AST against CP induced male germ cell toxicity was evaluated using body weight, testes and epididymis weight, sperm count, sperm head morphology, sperm comet assay, histology of testes and TUNEL assay. AST treatment significantly improved the testes weight, sperm count and sperm head morphology as compared to only CP treated animals. The result of comet assay showed that AST treatment significantly restored the sperm DNA damage induced by CP. Further, AST treatment showed protection against CP induced testicular toxicity as evident from testes histology and TUNEL assay. The present results indicate the chemoprotective potential of AST against CP induced germ cell toxicity in mice.
机译：环磷酰胺（CP），一种烷基化剂，用于治疗多种癌症以及类风湿关节炎的免疫抑制剂。由于其广谱的功效，它可用于抗多种癌症，但同时具有职业暴露以及对患者的治疗相关毒性的有害风险。本研究旨在研究虾青素（AST）一种红色类胡萝卜素色素对CP诱导的雄性小鼠生殖细胞毒性的保护作用。每周一次以50、100和200mg / kg体重的剂量腹膜内（i.p.）给予小鼠（20-25 g）一次，持续5周。 AST的剂量是每星期口服（p.o.）25mg / kg，连续五天，连续五周。最后一次注射CP后一周将动物处死。使用体重，睾丸和附睾重量，精子计数，精子头部形态，精子彗星试验，睾丸组织学和TUNEL试验评估AST对CP诱导的雄性生殖细胞毒性的保护作用。与仅CP治疗的动物相比，AST治疗显着改善了睾丸重量，精子数量和精子头部形态。彗星试验的结果表明，AST处理可显着恢复CP诱导的精子DNA损伤。此外，从睾丸组织学和TUNEL分析中可以明显看出，AST治疗对CP诱导的睾丸毒性具有保护作用。目前的结果表明AST对CP诱导的小鼠生殖细胞毒性的化学保护潜力。

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