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首页> 外文期刊>Toxicon: An International Journal Devoted to the Exchange of Knowledge on the Poisons Derived from Animals, Plants and Microorganisms >Surface plasmon resonance detection of ricin and horticultural ricin variants in environmental samples.
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Surface plasmon resonance detection of ricin and horticultural ricin variants in environmental samples.

机译：环境样品中蓖麻毒素和园艺蓖麻毒素变体的表面等离子体共振检测。

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摘要

A rapid, sensitive and robust immunoassay based on a commercial surface plasmon resonance (SPR) instrument (Biacore X) was developed for the detection of ricin in environmental samples. A total of 10 monoclonal antibodies were evaluated for their ability to recognise both a commercial ricin and horticultural ricin variants extracted from six different cultivars of Ricinus communis. Two suitable antibodies (7G12 and TFTA) were identified because of their strong affinity to all six ricin variants. The antibody 7G12 was used as the capture ligand in the SPR system. The assay was linear over a wide range of ricin concentrations (up to at least 750ng/ml) with a limit of detection of 0.5ng/ml. The assay was highly reproducible (coefficient of variation was less than 5%), and was able to detect all six ricin variants and environmental samples.
机译：基于商业表面等离振子共振（SPR）仪器（Biacore X）的快速，灵敏和鲁棒的免疫测定技术被开发用于检测环境样品中的蓖麻毒素。评估了总共10种单克隆抗体识别从蓖麻（Ricinus communis）的六个不同品种中提取的商业蓖麻毒素和园艺蓖麻毒素变体的能力。鉴定了两种合适的抗体（7G12和TFTA），因为它们对所有六个蓖麻毒蛋白变体都有很强的亲和力。抗体7G12用作SPR系统中的捕获配体。该测定在多种蓖麻毒素浓度（最高至少750ng / ml）内呈线性，检出限为0.5ng / ml。该测定具有高度可重复性（变异系数小于5％），并且能够检测所有六个蓖麻毒素变体和环境样品。

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